Anti-MED12 Antibody Picoband™

Product Name

Anti-MED12 Antibody Picoband™

View all MED12 Antibodies

SKU/Catalog Number

A00828-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-MED12 Antibody Picoband™ catalog # A00828-2. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-MED12 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00828-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MED12 recombinant protein (Position: Q752-Q1974).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A00828-2 is reactive to MED12 in Human

Applications

A00828-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

260 kDa

Calculated molecular weight

243.081kDa

Background of MED12

Mediator of RNA polymerase II transcription, subunit 12 homolog (S. cerevisiae), also known as MED12, is a human gene found on the X chromosome. The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of MED12 using anti-MED12 antibody (A00828-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED12 antigen affinity purified polyclonal antibody (Catalog # A00828-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED12 at approximately 260 kDa. The expected band size for MED12 is at 260 kDa.

Gene/Protein Information For MED12 (Source: Uniprot.org, NCBI)

Alternative Names

ARC240; CAG repeat protein 45; CAGH45trinucleotide repeat containing 11 (THR-associated protein, 230kDa subunit); HOPAFGS1; KIAA0192FG syndrome 1; mediator complex subunit 12OPA-containing protein; OKSsubunit 12 homolog; thyroid hormone receptor-associated protein, 230 kDa subunit; Trap230; TRAP230mediator of RNA polymerase II transcription, subunit 12 homolog (S. cerevisiae); trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit); Trinucleotide repeat-containing gene 11 protein MED12 ARC240, CAGH45, FGS1, HOPA, KtoS, OHDOX, OKS, OPA1, TNRC11, TRAP230, MED12 mediator complex subunit 12 mediator of RNA polymerase II transcription subunit 12|CAG repeat protein 45|Kohtalo homolog|OPA-containing protein|activator-recruited cofactor 240 kDa component|human opposite paired|mediator of RNA polymerase II transcription, subunit 12 homolog|putative mediator subunit 12|thyroid hormone receptor-associated protein complex 230 kDa component|thyroid hormone receptor-associated protein, 230 kDa subunit|trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit)|trinucleotide repeat-containing gene 11 protein

For more info on MED12, check out the MED12 Infographic

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In this infographic, you will see the following information for MED12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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