Anti-MED14/Crsp2 Antibody

MED14 antibody

Boster Bio Anti-MED14/Crsp2 Antibody (Catalog# A04799-1). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A04799-1
Size: 100ul
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, IF, IHC-P, ICC

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Product Name

Anti-MED14/Crsp2 Antibody

View all MED14 Antibodies

SKU/Catalog Number

A04799-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-MED14/Crsp2 Antibody (Catalog# A04799-1). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MED14/Crsp2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04799-1)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

7A7

Isotype

Rabbit IgG

Immunogen

A peptide derived from human MED14. Immunogen sequence location: 701-750

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A04799-1 is reactive to MED14 in Human, Mouse

Applications

A04799-1 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

160.607kDa

Background of MED14

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:40000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For MED14 (Source: Uniprot.org, NCBI)

Gene Name

MED14

Full Name

Mediator of RNA polymerase II transcription subunit 14

Weight

160.607kDa

Superfamily

Mediator complex subunit 14 family

Alternative Names

Activator-recruited cofactor 150 kDa component; ARC150; Cofactor required for Sp1 transcriptional activation subunit 2; cofactor required for Sp1 transcriptional activation, subunit 2 (150kD); CRSP150; CRSP2cofactor required for Sp1 transcriptional activation, subunit 2, 150kDa; CSRP; CXorf4MGC104513; EXLM1CRSP complex subunit 2; human homolog of yeast RGR1; mediator complex subunit 14DRIP150; mediator of RNA polymerase II transcription subunit 14; RGR1RGR1 homolog; Thyroid hormone receptor-associated protein complex 170 kDa component; thyroid hormone receptor-associated protein complex compon MED14 CRSP150, CRSP2, CSRP, CXorf4, DRIP150, EXLM1, RGR1, TRAP170 mediator complex subunit 14 mediator of RNA polymerase II transcription subunit 14|ARC150|CRSP complex subunit 2|RGR1 homolog|activator-recruited cofactor 150 kDa component|cofactor required for Sp1 transcriptional activation, subunit 2 (150kD)|hRGR1|human homolog of yeast RGR1|thyroid hormone receptor-associated protein complex 170 kDa component|thyroid hormone receptor-associated protein complex component TRAP170|transcriptional co-activator CRSP150|vitamin D receptor-interacting protein complex component DRIP150|vitamin D3 receptor-interacting protein complex 150 kDa component

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MED14, check out the MED14 Infographic

MED14 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MED14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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12 Customer Q&As for Anti-MED14/Crsp2 Antibody

Question

See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-MED14/Crsp2 antibody A04799-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-30

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-30

Question

Is a blocking peptide available for product anti-MED14/Crsp2 antibody (A04799-1)?

Verified Customer

Verified customer

Asked: 2020-01-20

Answer

We do provide the blocking peptide for product anti-MED14/Crsp2 antibody (A04799-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-20

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-MED14/Crsp2 antibody A04799-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-20

Question

Will anti-MED14/Crsp2 antibody A04799-1 work for IHC with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

According to the expression profile of cervix carcinoma erythroleukemia, MED14 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-MED14/Crsp2 antibody A04799-1 will work for IHC with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2019-08-26

Question

I see that the anti-MED14/Crsp2 antibody A04799-1 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-27

Question

Is this A04799-1 anti-MED14/Crsp2 antibody reactive to the isotypes of MED14?

Verified Customer

Verified customer

Asked: 2019-06-13

Answer

The immunogen of A04799-1 anti-MED14/Crsp2 antibody is A peptide derived from human MED14. Immunogen sequence location: 701-750. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-13

Question

Does A04799-1 anti-MED14/Crsp2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

C. Jackson

Verified customer

Asked: 2019-04-19

Answer

It shows on the product datasheet, A04799-1 anti-MED14/Crsp2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-04-19

Question

My question regarding product A04799-1, anti-MED14/Crsp2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-06-18

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04799-1 anti-MED14/Crsp2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-06-18

Question

Will anti-MED14/Crsp2 antibody A04799-1 work on horse IHC with secondary oocyte?

Verified Customer

Verified customer

Asked: 2017-06-30

Answer

Our lab technicians have not validated anti-MED14/Crsp2 antibody A04799-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-MED14/Crsp2 antibody A04799-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse secondary oocyte in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-06-30

Question

I was wanting to use your anti-MED14/Crsp2 antibody for IHC for mouse cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma erythroleukemia identification?

C. Jones

Verified customer

Asked: 2016-09-20

Answer

As indicated on the product datasheet, A04799-1 anti-MED14/Crsp2 antibody has been tested for IF, IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-09-20

Question

Do you have a BSA free version of anti-MED14/Crsp2 antibody A04799-1 available?

S. Mangal

Verified customer

Asked: 2014-02-18

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MED14/Crsp2 antibody A04799-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-02-18

Question

you antibody to test anti-MED14/Crsp2 antibody A04799-1 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-MED14/Crsp2 antibody A04799-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Z. Baker

Verified customer

Asked: 2013-03-11

Answer

The products we sell, including anti-MED14/Crsp2 antibody A04799-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-03-11

Order DetailsPrice
A04799-1

100 μL

$335

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