Anti-MED14 Antibody Picoband™

MED14 antibody

Boster Bio Anti-MED14 Antibody Picoband™ catalog # A04799-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A04799-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Customers Who Bought This Also Bought

Product Name

Anti-MED14 Antibody Picoband™

View all MED14 Antibodies

SKU/Catalog Number

A04799-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MED14 Antibody Picoband™ catalog # A04799-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MED14 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04799-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human MED14 recombinant protein (Position: D181-D375).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04799-2 is reactive to MED14 in Human, Mouse, Rat

Applications

A04799-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

160 kDa

Calculated molecular weight

160.607kDa

Background of MED14

Mediator of RNA polymerase II transcription subunit 14 is an enzyme that in humans is encoded by the MED14 gene. The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For MED14 (Source: Uniprot.org, NCBI)

Gene Name

MED14

Full Name

Mediator of RNA polymerase II transcription subunit 14

Weight

160.607kDa

Superfamily

Mediator complex subunit 14 family

Alternative Names

Activator-recruited cofactor 150 kDa component; ARC150; Cofactor required for Sp1 transcriptional activation subunit 2; cofactor required for Sp1 transcriptional activation, subunit 2 (150kD); CRSP150; CRSP2cofactor required for Sp1 transcriptional activation, subunit 2, 150kDa; CSRP; CXorf4MGC104513; EXLM1CRSP complex subunit 2; human homolog of yeast RGR1; mediator complex subunit 14DRIP150; mediator of RNA polymerase II transcription subunit 14; RGR1RGR1 homolog; Thyroid hormone receptor-associated protein complex 170 kDa component; thyroid hormone receptor-associated protein complex compon MED14 CRSP150, CRSP2, CSRP, CXorf4, DRIP150, EXLM1, RGR1, TRAP170 mediator complex subunit 14 mediator of RNA polymerase II transcription subunit 14|ARC150|CRSP complex subunit 2|RGR1 homolog|activator-recruited cofactor 150 kDa component|cofactor required for Sp1 transcriptional activation, subunit 2 (150kD)|hRGR1|human homolog of yeast RGR1|thyroid hormone receptor-associated protein complex 170 kDa component|thyroid hormone receptor-associated protein complex component TRAP170|transcriptional co-activator CRSP150|vitamin D receptor-interacting protein complex component DRIP150|vitamin D3 receptor-interacting protein complex 150 kDa component

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MED14, check out the MED14 Infographic

MED14 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MED14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A04799-2

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-MED14 Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-MED14 Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

1 Customer Q&As for Anti-MED14 Antibody Picoband™

Question

We are currently using anti-MED14 antibody A04799-2 for rat tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

The anti-MED14 antibody (A04799-2) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-03

Order DetailsPrice
A04799-2

100μg

$370
A04799-2-10ug

10μg sample (liquid)

$99
A04799-2-Biotin

100 μg Biotin conjugated

$570
A04799-2-Cy3

100 μg Cy3 conjugated

$570
A04799-2-Dylight488

100 μg Dylight488 conjugated

$570
A04799-2-Dylight550

100 μg Dylight550 conjugated

$570
A04799-2-Dylight594

100 μg Dylight594 conjugated

$570
A04799-2-FITC

100 μg FITC conjugated

$570
A04799-2-HRP

100 μg HRP conjugated

$570
A04799-2-APC

100 μg APC conjugated

$670
A04799-2-PE

100 μg PE conjugated

$670
A04799-2-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
A04799-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.