Anti-MED28 Antibody

Product Name

Anti-MED28 Antibody

View all MED28 Antibodies

SKU/Catalog Number

A08611

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-MED28 Antibody (Catalog # A08611). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.

Storage & Handling

MED28 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-MED28 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08611)

Host

Rabbit

Contents

MED28 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

MED28 antibody was raised against a 17 amino acid synthetic peptide from near the center of human MED28. The immunogen is located within amino acids 50 - 100 of MED28.

Reactive Species

A08611 is reactive to MED28 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

19.5 kDa

Background of MED28

The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED28, also known as Magicin. MED28 is expressed in many cell lines and tissues. It has been shown that a down-regulation of MED28 expression in NIH3T3 cells results in a significant induction of several genes associated with smooth muscle cell differentiation and conversely its over-expression represses expression of SMC genes. MED28 can also form a ternary complex with Grb2 and Merlin, the neurofibromatosis 2 tumor suppressor protein, indicating that MED28 may play a role in Merlin's tumor suppressive activity. MED28 has also been recently identified as an HIV dependency factor (HDF), suggesting that MED28 may be an important drug target in HIV treatment. At least two isoforms of MED28 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08611 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

MED28 antibody can be used for detection of MED28 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information MED28

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