Anti-MED29 Antibody Picoband™

Product Name

Anti-MED29 Antibody Picoband™

View all MED29 Antibodies

SKU/Catalog Number

A10590-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-MED29 Antibody Picoband™ catalog # A10590-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-MED29 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10590-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MED29 recombinant protein (Position: Q29-K184).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10590-1 is reactive to MED29 in Human

Applications

A10590-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

21 kDa

Calculated molecular weight

21.073kDa

Background of MED29

Mediator of RNA polymerase II transcription subunit 29 (Med29) is a transcription suppressor that in humans is encoded by the MED29 gene. MED29 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II and general initiation factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of MED29 using anti-MED29 antibody (A10590-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Siha whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: human U251 whole cell lysates,
Lane 7: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED29 antigen affinity purified polyclonal antibody (Catalog # A10590-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED29 at approximately 21 kDa. The expected band size for MED29 is at 21 kDa.

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Figure 2. IF analysis of MED29 using anti-MED29 antibody (A10590-1) and anti-Beta Tubulin antibody (M01857-3).
MED29 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MED29 Antibody (A10590-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For MED29 (Source: Uniprot.org, NCBI)

Alternative Names

intersex-like (Drosophila); Intersex-like protein; mediator complex subunit 29IXLDKFZp434H247; mediator of RNA polymerase II transcription subunit 29 MED29 IXL, MED2 mediator complex subunit 29 mediator of RNA polymerase II transcription subunit 29|intersex-like protein

For more info on MED29, check out the MED29 Infographic

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In this infographic, you will see the following information for MED29: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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