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Product Info Summary
| SKU: | M01602 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-MEIS1 Monoclonal Antibody
SKU/Catalog Number
M01602
BM5247 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MEIS1 Monoclonal Antibody catalog # M01602. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEIS1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01602)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACDO-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MEIS1
Reactive Species
M01602 is reactive to MEIS1 in Human, Mouse, Rat
Observed Molecular Weight
45,53 kDa
Calculated molecular weight
43.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01602 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:20
FC 1:20
Positive Control
WB: human K562 whole cell, mouse pancrease tissue, human SH-SY5Y whole cell, human Hela whole cell, human HEL whole cell
Validation Images & Assay Conditions
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Western blot analysis of MEIS1 using anti-MEIS1 antibody (M01602).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEIS1 antigen affinity purified monoclonal antibody (M01602) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MEIS1 at approximately 45,53 kDa. The expected band size for MEIS1 is at 43 kDa.
Click image to see more details
Western blot analysis of MEIS1 using anti-MEIS1 antibody (M01602).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEIS1 antigen affinity purified monoclonal antibody (M01602) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MEIS1 at approximately 45,53 kDa. The expected band size for MEIS1 is at 43 kDa.
Specific Publications For Anti-MEIS1 Monoclonal Antibody (M01602)
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Customer Q&As
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7 Customer Q&As for Anti-MEIS1 Monoclonal Antibody
Question
Does anti-MEIS1 Monoclonal antibody M01602 work for Flow Cytometry with placenta?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
According to the expression profile of placenta, MEIS1 is highly expressed in placenta. So, it is likely that anti-MEIS1 Monoclonal antibody M01602 will work for Flow Cytometry with placenta.
Boster Scientific Support
Answered: 2019-12-17
Question
We are currently using anti-MEIS1 Monoclonal antibody M01602 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
The anti-MEIS1 Monoclonal antibody (M01602) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-01
Question
Can you help my question with product M01602, anti-MEIS1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01602 anti-MEIS1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-07
Question
I have attached the WB image, lot number and protocol we used for placenta using anti-MEIS1 Monoclonal antibody M01602. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-31
Question
Will anti-MEIS1 Monoclonal antibody M01602 work on primate WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-03-05
Answer
Our lab technicians have not validated anti-MEIS1 Monoclonal antibody M01602 on primate. You can run a BLAST between primate and the immunogen sequence of anti-MEIS1 Monoclonal antibody M01602 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-05
Question
My question regards to test anti-MEIS1 Monoclonal antibody M01602 on rat placenta for research purposes, then I may be interested in using anti-MEIS1 Monoclonal antibody M01602 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Jackson
Verified customer
Asked: 2017-07-12
Answer
The products we sell, including anti-MEIS1 Monoclonal antibody M01602, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-12
Question
I was wanting to use your anti-MEIS1 Monoclonal antibody for Flow Cytometry for rat placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat placenta identification?
J. Williams
Verified customer
Asked: 2013-11-19
Answer
It shows on the product datasheet, M01602 anti-MEIS1 Monoclonal antibody has been tested for Flow Cytometry, IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-11-19
