Anti-MEK1 phosphoS222/MEK2 phosphoS226 Monoclonal Antibody

Product Name

Anti-MEK1 phosphoS222/MEK2 phosphoS226 Monoclonal Antibody

View all MAP2K1/MAP2K2 Antibodies

SKU/Catalog Number

MP00292

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-MEK1 phosphoS222/MEK2 phosphoS226 Monoclonal Antibody (Catalog # MP00292). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store vial at -20°C prior to opening. This product is stable at 4°C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-MEK1 phosphoS222/MEK2 phosphoS226 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP00292)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 17C9.E1.H2.D7.H7.D5

Isotype

IgG1 lambda

Immunogen

Anti-MEK1 pS222/MEK2 pS226 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues surrounding the S222-226 phosphorylation site conjugated to KLH.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

MP00292 is reactive to MAP2K1/MAP2K2 in Human, Mouse, Rat

Observed Molecular Weight

42 kDa

Calculated molecular weight

43.4 kDa

Background of MAP2K1/MAP2K2

MEK1 pS222/MEK2 pS226 antibody detects MEK1 and MEK2. Mitogen-activated protein kinase kinase 1, (also known as MKK or MEK1), and Mitogen-activated protein kinase kinase 2, (also known as MEK2 or MKK2), are integral components of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 and 2 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. The MEK1 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

MP00292 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:40,000
WB: User optimized
Anti-MEK1 pS222/MEK2 pS226 (MOUSE) antibody has been tested in ELISA and Western Blot. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa.

Validation Images & Assay Conditions

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See full target information MAP2K1/MAP2K2

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