Anti-MEK2 C-Term MAP2K2 Monoclonal Antibody Biotin

Product Name

Anti-MEK2 C-Term MAP2K2 Monoclonal Antibody Biotin

View all MEK2 Antibodies

SKU/Catalog Number

B00996

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-MEK2 C-Term MAP2K2 Monoclonal Antibody Biotin (Catalog # B00996). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-MEK2 C-Term MAP2K2 Monoclonal Antibody Biotin (Boster Biological Technology, Pleasanton CA, USA, Catalog # B00996)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 12A6.G1.G11 IgG1 kappa

Isotype

IgG1 kappa

Immunogen

Anti-MEK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

B00996 is reactive to MAP2K2 in Human, Mouse, Rat

Applications

B00996 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

44.424kDa

Background of MEK2

MEK2 antibodies detect the MEK2 isoform. Mitogen-activated protein kinase kinase 2, also known as MEK2 or MKK2, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 2 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. MEK1 and MEK2 are about 80% identical to each other, and nearly identical within the kinase domain. This antibody does not react with MEK1. The MEK2 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:40,000
Fluorochrome Protein Value: 10-20
WB: 1µg/mL

Validation Images & Assay Conditions

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Western blot analysis of MEK2 in 50 ng MEK-1 recombinant protein (lane 1) and 50 ng MEK-2 recombinant protein (lane 2) using mouse anti-MEK2 affinity purified monoclonal antibody (Catalog # B00996). The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).

Gene/Protein Information For MAP2K2 (Source: Uniprot.org, NCBI)

Alternative Names

ERK activator kinase 2; MAP2K2; MAPKK 2; MEK 2; MEK2; MEK2EC 2.7.12.2; mitogen-activated protein kinase kinase 2; MKK2; MKK2FLJ26075; p45; PRKMK2MAPKK2 MAP2K2 CFC4, MAPKK2, MEK2, MKK2, PRKMK2 mitogen-activated protein kinase kinase 2 dual specificity mitogen-activated protein kinase kinase 2|ERK activator kinase 2|MAP kinase kinase 2|MAPK/ERK kinase 2|mitogen-activated protein kinase kinase 2, p45

For more info on MAP2K2, check out the MAP2K2 Infographic

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In this infographic, you will see the following information for MAP2K2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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