|Validated Species:||Mouse, Rat|
Data & Images
|Product Name||Anti-MEKK3 Antibody|
|Description||Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 3(MAP3K3) detection. Tested with WB, IHC-P in Mouse;Rat.|
|Cite This Product||Anti-MEKK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1925)|
|Replacement Item||This antibody may replace the following items: sc-1089|sc-136260|sc-28043-R|sc-28044|sc-28044-R|sc-28769|sc-398091|sc-6843|sc-6844 from Santa Cruz Biotechnology.|
|Validated Species||Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of mouse MEKK3(11-25aa MKDLVALQMSRRTRL), different from the related rat sequence by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Mitogen-activated protein kinase kinase kinase 3|
|Molecular Weight||70776 MW|
|Protein Function||Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. .|
|Sequence Similarities||Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.|
|Alternative Names||Mitogen-activated protein kinase kinase kinase 3;188.8.131.52;MAPK/ERK kinase kinase 3;MEK kinase 3;MEKK 3;Map3k3;Mekk3;|
|Research Areas|||signal transduction|protein phosphorylation|ser / thr kinases|mapk pathway| immunology|innate immunity|tlr signaling||
Background for Mitogen-activated protein kinase kinase kinase 3
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MEKK3 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MEKK3 Antibody Images
Click the images to enlarge.
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
All lanes: Anti MAP3K3 (PA1925) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 71KD
Observed bind size: 71KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,