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MUT antibody (M01065) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody (M01065).
Methylmalonyl Coenzyme A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Methylmalonyl Coenzyme A Antibody (M01065) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Methylmalonyl Coenzyme A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Methylmalonyl Coenzyme A Antibody (M01065) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband® (monoclonal, 2D6)
MUT antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | IHC, WB |
Tested Applications
MUT antibody was positively detected by WB in:
human Hela whole cell,, human K562 whole cell,, human HEK293 whole cell,, human PC-3 whole cell,, human Caco-2 whole cell,, human Raji whole cell,, rat PC-12 whole cell,, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MUT antibody was positively detected by IHC in:
human mammary cancer tissue, human rectal cancer tissue, human rectal cancer tissue, rat intestine tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband® (monoclonal, 2D6)
View all MUT Antibodies
View all MUT Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
2D6
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MUT, different from the related mouse sequence by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
83 kDa
Calculated Molecular Weight
83.1 kDa
Background of MUT
Methylmalonyl-CoA mutase (MUT) is a mitochondrial enzyme that catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA. This gene is mapped to 6p12.3. MUT requires a vitamin B12-derived prosthetic group, adenosylcobalamin (commonly referred to as AdoCbl), to function. And the product of this enzyme, succinyl-CoA, is a key molecule of the TCA cycle.
Cite This Product
Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband® (monoclonal, 2D6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01065)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Mouse
97%
High Homology
Rat
97%
High Homology
Rabbit
97%
High Homology
Cow
97%
High Homology
Sheep
97%
High Homology
Zebrafish
73%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MUT antibody
Immunohistochemistry (IHC) protocol for MUT antibody
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Product Promise
We promise Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband® (monoclonal, 2D6) performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M01065
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026