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Product Info Summary
| SKU: | A04894 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Methylosome protein 50 WDR77 Antibody
SKU/Catalog Number
A04894
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Methylosome protein 50 WDR77 Antibody catalog # A04894. Tested in WB,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Methylosome protein 50 WDR77 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04894)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human WDR77(NP_077007.1).
Reactive Species
A04894 is reactive to WDR77 in Human, Mouse, Rat
Observed Molecular Weight
111 kDa
Calculated molecular weight
36.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04894 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
ICC/IF: 1:50-1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using WDR77 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
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Immunofluorescence analysis of A-549 cells using WDR77 antibody . Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of Mouse brain, using WDR77 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.
Specific Publications For Anti-Methylosome protein 50 WDR77 Antibody (A04894)
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