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Product Info Summary
| SKU: | A11383-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-METRNL Antibody Picoband®
SKU/Catalog Number
A11383-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-METRNL Antibody Picoband® catalog # A11383-1. Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-METRNL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11383-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human METRNL recombinant protein (Position: K66-D311). Human METRNL shares 78.5% and 79.3% amino acid (aa) sequence identity with mouse and rat METRNL, respectively.
Reactive Species
A11383-1 is reactive to METRNL in Human
Observed Molecular Weight
34 kDa
Calculated molecular weight
34.4 kDa
Background of METRNL
Meteorin-like/Meteorin-Beta (Metrnl)/IL-41, also known as subfatin and cometin, is a small (~27kDa) secreted cytokine, protein encoded by a gene called meteorin-like (METRNL). Meteorin-like protein (METRNL) is a 311 amino acid (aa) protein that shows 45% homology with Meteorin (in mouse). Meteorin itself is a secreted 30 kDa protein that is expressed in glial and neuronal cells and their precursors during development and in the adult. In the adult Meteorin is primarily expressed by astrocytes. Meteorin promotes neurite outgrowth, glial cell differentiation, and neuronal survival following excitotoxic injury. It also inhibits sprouting angiogenesis and promotes blood vessel maturation. Mouse Meteorin?like protein is synthesized with a 45 amino acid (aa) signal peptide and is selectively expressed in the otic vesicle during inner ear development. Mature mouse Meteorin?like protein shares 79% and 98% aa sequence identity with human and rat Meteorin?like protein, respectively. Alternate splicing generates an additional isoform that lacks the N?terminal 82 amino acids including the signal peptide. The promoter region of the METRNL gene contains a recognition site for the Pax2, Pax5, and Pax8 transcription factors, and Pax2 binds specifically to this region. METRNL is the only gene found within the terminus of the human chromosome 17 q?arm which can be deleted in the rare Ring 17 syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11383-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human HEL whole cell, human MCF-7 whole cell
Validation Images & Assay Conditions
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Western blot analysis of Meteorin-like/METRNL using anti-Meteorin-like/METRNL antibody (A11383-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Meteorin-like/METRNL antigen affinity purified polyclonal antibody (Catalog # A11383-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Meteorin-like/METRNL at approximately 34 kDa. The expected band size for Meteorin-like/METRNL is at 34 kDa.
Specific Publications For Anti-METRNL Antibody Picoband® (A11383-1)
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