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Product Info Summary
| SKU: | A01758-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-METTL3 Antibody Picoband®
SKU/Catalog Number
A01758-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-METTL3/METTL3 Antibody Picoband® catalog # A01758-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-METTL3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01758-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human METTL3 recombinant protein (Position: Q423-D571).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01758-1 is reactive to METTL3 in Human, Mouse, Rat
Observed Molecular Weight
64 kDa
Calculated molecular weight
64.5 kDa
Background of METTL3
N6-adenosine-methyltransferase 70 kDa subunit (METTL3) is an enzyme that in humans is encoded by theMETTL3 gene. It is mapped to 14q11.2. This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01758-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human HepG2 whole cell, human PC-3 whole cell, human A549 whole cell, human Hacat whole cell, rat brain tissue, rat testis tissue, mouse brain tissue
FCM: HEPA1-6 cell
Validation Images & Assay Conditions
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Flow Cytometry analysis of HEPA1-6 cells using anti-METTL3 antibody (A01758-1).
Overlay histogram showing HEPA1-6 cells stained with A01758-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-METTL3 Antibody (A01758-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Western blot analysis of METTL3 using anti-METTL3 antibody (A01758-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human Hacat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL3 antigen affinity purified polyclonal antibody (Catalog # A01758-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for METTL3 at approximately 64 kDa. The expected band size for METTL3 is at 64 kDa.
Specific Publications For Anti-METTL3 Antibody Picoband® (A01758-1)
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Customer Q&As
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5 Customer Q&As for Anti-METTL3 Antibody Picoband®
Question
Is this A01758-1 anti-METTL3/METTL3 antibody reactive to the isotypes of METTL3?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
The immunogen of A01758-1 anti-METTL3/METTL3 antibody is E. coli-derived human METTL3 recombinant protein (Position: Q423-D571). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-20
Question
We are currently using anti-METTL3/METTL3 antibody A01758-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
The anti-METTL3/METTL3 antibody (A01758-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-02
Question
Would anti-METTL3/METTL3 antibody A01758-1 work for ICC with endometrium?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
According to the expression profile of endometrium, METTL3 is highly expressed in endometrium. So, it is likely that anti-METTL3/METTL3 antibody A01758-1 will work for ICC with endometrium.
Boster Scientific Support
Answered: 2018-12-18
Question
I see that the anti-METTL3/METTL3 antibody A01758-1 works with ICC, what is the protocol used to produce the result images on the product page?
J. Moore
Verified customer
Asked: 2015-06-18
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-06-18
Question
Does anti-METTL3/METTL3 antibody A01758-1 work on pig ICC with cervix carcinoma erythroleukemia?
K. Miller
Verified customer
Asked: 2015-03-23
Answer
Our lab technicians have not tested anti-METTL3/METTL3 antibody A01758-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-METTL3/METTL3 antibody A01758-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma erythroleukemia in ICC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-23
