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Product Info Summary
| SKU: | A16078-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MFSD4A Antibody Picoband®
SKU/Catalog Number
A16078-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-MFSD4A Antibody Picoband® catalog # A16078-1. Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MFSD4A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16078-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human MFSD4A recombinant protein (Position: S48-R514). Human MFSD4A shares 85.7% amino acid (aa) sequence identity with mouse MFSD4A.
Reactive Species
A16078-1 is reactive to MFSD4A in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
56.3 kDa
Background of MFSD4A
Major facilitator superfamily domain containing 4A is a protein belonging to the MFS Pfam clan. It is an atypical solute carrier, thus a plausible SLC transporter in humans. MFSD4A has been identified in both central and peripheral areas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16078-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human PC-3 whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
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Western blot analysis of MFSD4A using anti-MFSD4A antibody (A16078-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human HepG2 whole cell lysates
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFSD4A antigen affinity purified polyclonal antibody (Catalog # A16078-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MFSD4A at approximately 68 kDa. The expected band size for MFSD4A is at 56 kDa.
Specific Publications For Anti-MFSD4A Antibody Picoband® (A16078-1)
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