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Product Info Summary
| SKU: | A06163-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-mGluR6 GRM6 Antibody
SKU/Catalog Number
A06163-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-mGluR6 GRM6 Antibody (Catalog# A06163-1). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-mGluR6 GRM6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06163-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human mGluR6. Immunogen sequence location: 828-877
Reactive Species
A06163-1 is reactive to GRM6 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
95.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06163-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using mGluR6 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from mouse brain, using mGluR6 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-mGluR6 GRM6 Antibody (A06163-1)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-mGluR6 GRM6 Antibody
Question
Will anti-mGluR6 antibody A06163-1 work for IHC with gastrocnemius?
G. Jha
Verified customer
Asked: 2020-04-29
Answer
According to the expression profile of gastrocnemius, GRM6 is highly expressed in gastrocnemius. So, it is likely that anti-mGluR6 antibody A06163-1 will work for IHC with gastrocnemius.
Boster Scientific Support
Answered: 2020-04-29
Question
Does A06163-1 anti-mGluR6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
You can see on the product datasheet, A06163-1 anti-mGluR6 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-24
Question
We are currently using anti-mGluR6 antibody A06163-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-07
Answer
The anti-mGluR6 antibody (A06163-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-07
