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Product Info Summary
| SKU: | M01012 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MGMT Rabbit Monoclonal Antibody
SKU/Catalog Number
M01012
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MGMT Rabbit Monoclonal Antibody catalog # M01012. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MGMT Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01012)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IDD-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MGMT
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01012 is reactive to MGMT in Human
Applications
M01012 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
22 kDa
Calculated molecular weight
21.646kDa
Background of MGMT
Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of MGMT expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MGMT (Source: Uniprot.org, NCBI)
Gene Name
MGMT
Full Name
Methylated-DNA--protein-cysteine methyltransferase
Weight
21.646kDa
Superfamily
MGMT family
Alternative Names
6-O-methylguanine-DNA methyltransferase; EC 2.1.1.63; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA methyltransferase; O6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase MGMT O-6-methylguanine-DNA methyltransferase methylated-DNA--protein-cysteine methyltransferase|6-O-methylguanine-DNA methyltransferase|O-6-methylguanine-DNA-alkyltransferase|O6-methylguanine-DNA methyltransferase|methylguanine-DNA methyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MGMT, check out the MGMT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MGMT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MGMT Rabbit Monoclonal Antibody (M01012)
Hello CJ!
M01012 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Minea RO,Duc TC,Swenson SD,Cho HY,Huang M,Hartman H,Hofman FM,Schönthal AH,Chen TC.Developing a clinically relevant radiosensitizer for temozolomide-resistant gliomas.PLoS One.2020 Sep 3;15(9):e0238238.doi:10.1371/journal.pone.0238238.PMID:32881880;PMCID:
Species: Human,Mouse
M01012 usage in article: APP:WB, SAMPLE:HUMAN GB CELL LINES, DILUTION:NA
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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17 Customer Q&As for Anti-MGMT Rabbit Monoclonal Antibody
Question
My question regarding product M01012, anti-MGMT Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01012 anti-MGMT Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-29
Question
I am looking for using your anti-MGMT Rabbit Monoclonal antibody for mgmt-mediated dna damage reversal studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
Thank you for your inquiry. This M01012 anti-MGMT Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-13
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-MGMT Rabbit Monoclonal antibody M01012. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-10
Question
I was wanting to use your anti-MGMT Rabbit Monoclonal antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
As indicated on the product datasheet, M01012 anti-MGMT Rabbit Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-13
Question
Is a blocking peptide available for product anti-MGMT Rabbit Monoclonal antibody (M01012)?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We do provide the blocking peptide for product anti-MGMT Rabbit Monoclonal antibody (M01012). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-09
Question
We have observed staining in human placenta. What should we do? Is anti-MGMT Rabbit Monoclonal antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
Based on literature placenta does express MGMT. Based on Uniprot.org, MGMT is expressed in liver, placenta, cervix carcinoma, among other tissues. Regarding which tissues have MGMT expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-26
Question
For
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The immunogen sequence of CY5981 is CEMKRTTLDSPLGKLE.
Boster Scientific Support
Answered: 2019-11-14
Question
Does anti-MGMT Rabbit Monoclonal antibody M01012 work for WB with liver?
G. Wu
Verified customer
Asked: 2018-12-03
Answer
According to the expression profile of liver, MGMT is highly expressed in liver. So, it is likely that anti-MGMT Rabbit Monoclonal antibody M01012 will work for WB with liver.
Boster Scientific Support
Answered: 2018-12-03
Question
Does anti-MGMT Rabbit Monoclonal antibody M01012 work on monkey WB with placenta?
Verified Customer
Verified customer
Asked: 2018-03-07
Answer
Our lab technicians have not validated anti-MGMT Rabbit Monoclonal antibody M01012 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-MGMT Rabbit Monoclonal antibody M01012 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-07
Question
Would M01012 anti-MGMT Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
As indicated on the product datasheet, M01012 anti-MGMT Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-09
Question
I would like to test anti-MGMT Rabbit Monoclonal antibody M01012 on human liver for research purposes, then I may be interested in using anti-MGMT Rabbit Monoclonal antibody M01012 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Patel
Verified customer
Asked: 2017-03-02
Answer
The products we sell, including anti-MGMT Rabbit Monoclonal antibody M01012, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-03-02
Question
We are currently using anti-MGMT Rabbit Monoclonal antibody M01012 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
A. Carter
Verified customer
Asked: 2016-04-04
Answer
The anti-MGMT Rabbit Monoclonal antibody (M01012) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-04-04
Question
I see that the anti-MGMT Rabbit Monoclonal antibody M01012 works with WB, what is the protocol used to produce the result images on the product page?
E. Kulkarni
Verified customer
Asked: 2016-02-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-02-25
Question
My colleagues were content with the WB result of your anti-MGMT Rabbit Monoclonal antibody. However we have observed positive staining in liver nucleus. using this antibody. Is that expected? Could you tell me where is MGMT supposed to be expressed?
F. Zhao
Verified customer
Asked: 2016-02-15
Answer
According to literature, liver does express MGMT. Generally MGMT expresses in nucleus. Regarding which tissues have MGMT expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-02-15
Question
Is there a BSA free version of anti-MGMT Rabbit Monoclonal antibody M01012 available?
K. Moore
Verified customer
Asked: 2015-03-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MGMT Rabbit Monoclonal antibody M01012 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-03-04
Question
See attached the WB image, lot number and protocol we used for liver using anti-MGMT Rabbit Monoclonal antibody M01012. Please let me know if you require anything else.
R. Moore
Verified customer
Asked: 2014-09-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-09-24
Question
Is this M01012 anti-MGMT Rabbit Monoclonal antibody reactive to the isotypes of MGMT?
A. Lewis
Verified customer
Asked: 2013-03-04
Answer
The immunogen of M01012 anti-MGMT Rabbit Monoclonal antibody is A synthesized peptide derived from human MGMT. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-03-04
