Product Info Summary
SKU: | PB9723 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Microsomal Glutathione S-transferase 1/MGST1 Antibody Picoband™
View all Microsomal Glutathione S-transferase 1 Antibodies
SKU/Catalog Number
PB9723
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Microsomal Glutathione S-transferase 1/MGST1 Antibody Picoband™ catalog # PB9723. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Microsomal Glutathione S-transferase 1/MGST1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9723)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MGST1, different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9723 is reactive to MGST1 in Human, Mouse, Rat
Applications
PB9723 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
17 kDa
Calculated molecular weight
17.599kDa
Background of Microsomal Glutathione S-transferase 1
Microsomal glutathione S-transferase 1 is an enzyme that in humans is encoded by the MGST1 gene. The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Several transcript variants, some non-protein coding and some protein coding, have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MGST1 using anti-MGST1 antibody (PB9723).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Liver Tissue Lysate at 50ug,
Lane 2: Mouse Liver Tissue Lysate at 50ug,
Lane 3: HEPG2 Whole Cell Lysate at 40ug.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGST1 antigen affinity purified polyclonal antibody (Catalog # PB9723) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGST1 at approximately 17KD. The expected band size for MGST1 is at 17KD.
Protein Target Info & Infographic
Gene/Protein Information For MGST1 (Source: Uniprot.org, NCBI)
Gene Name
MGST1
Full Name
Microsomal glutathione S-transferase 1
Weight
17.599kDa
Superfamily
MAPEG family
Alternative Names
glutathione S-transferase 12; GST12EC 2.5.1.18; MGC14525; MGST; MGST-I; microsomal glutathione S-transferase 1; Microsomal GST-1; Microsomal GST-I MGST1 GST12, MGST, MGST-I microsomal glutathione S-transferase 1 microsomal glutathione S-transferase 1|glutathione S-transferase 12|microsomal GST-1|microsomal GST-I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MGST1, check out the MGST1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MGST1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Microsomal Glutathione S-transferase 1/MGST1 Antibody Picoband™ (PB9723)
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No publications found for PB9723
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Microsomal Glutathione S-transferase 1/MGST1 Antibody Picoband™
Question
I was wanting to use your anti-Microsomal Glutathione S-transferase 1/MGST1 antibody for WB for mouse liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
As indicated on the product datasheet, PB9723 anti-Microsomal Glutathione S-transferase 1/MGST1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-11
Question
My question regarding product PB9723, anti-Microsomal Glutathione S-transferase 1/MGST1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9723 anti-Microsomal Glutathione S-transferase 1/MGST1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-24
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Microsomal Glutathione S-transferase 1/MGST1 antibody PB9723. Let me know if you need anything else.
C. Kulkarni
Verified customer
Asked: 2019-04-30
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-30
Question
Is a blocking peptide available for product anti-Microsomal Glutathione S-transferase 1/MGST1 antibody (PB9723)?
Verified Customer
Verified customer
Asked: 2017-10-03
Answer
We do provide the blocking peptide for product anti-Microsomal Glutathione S-transferase 1/MGST1 antibody (PB9723). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-03
Question
We are currently using anti-Microsomal Glutathione S-transferase 1/MGST1 antibody PB9723 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
C. Krishna
Verified customer
Asked: 2015-06-12
Answer
The anti-Microsomal Glutathione S-transferase 1/MGST1 antibody (PB9723) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-06-12
Question
Will anti-Microsomal Glutathione S-transferase 1/MGST1 antibody PB9723 work on bovine WB with liver?
R. Yang
Verified customer
Asked: 2013-05-08
Answer
Our lab technicians have not validated anti-Microsomal Glutathione S-transferase 1/MGST1 antibody PB9723 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Microsomal Glutathione S-transferase 1/MGST1 antibody PB9723 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-05-08