Anti-Mi2- alpha Monoclonal Antibody

CHD3 antibody

Boster Bio Anti-Mi2- alpha Monoclonal Antibody catalog # A03200. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Pig, Rat.

Product Info Summary

SKU: A03200
Size: 100μl
Reactive Species: Bovine, Human, Mouse, Pig, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC, WB

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Product Name

Anti-Mi2- alpha Monoclonal Antibody

View all CHD3 Antibodies

SKU/Catalog Number

A03200

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Mi2- alpha Monoclonal Antibody catalog # A03200. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Mi2- alpha Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03200)

Host

Mouse

Contents

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Clonality

Monoclonal

Clone Number

1A4

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human CD39.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03200 is reactive to CHD3 in Bovine, Human, Mouse, Pig, Rat

Applications

A03200 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

226.592kDa

Background of CHD3

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 7 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 7 seems to be involved proteosomal degradation of proteins involved in endothelial proliferation and/or differentiation. Cullin 7 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1. Cullin 7 interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:1000-1:2000
IHC, 1:500-1:1000
IF, 1:100-1:500
FC, 1:100-1:200

Validation Images & Assay Conditions

Gene/Protein Information For CHD3 (Source: Uniprot.org, NCBI)

Gene Name

CHD3

Full Name

Chromodomain-helicase-DNA-binding protein 3

Weight

226.592kDa

Superfamily

SNF2/RAD54 helicase family

Alternative Names

ATP-dependent helicase CHD3; CHD-3; chromodomain helicase DNA binding protein 3; chromodomain-helicase-DNA-binding protein 3; EC 3.6.1; EC 3.6.4.12; hZFH; Mi-2 autoantigen 240 kDa protein; Mi-2a; Mi2-ALPHA; ZFH; Zinc finger helicase; zinc-finger helicase (Snf2-like) CHD3 Mi-2a, Mi2-ALPHA, SNIBCPS, ZFH chromodomain helicase DNA binding protein 3 chromodomain-helicase-DNA-binding protein 3|ATP-dependent helicase CHD3|CHD-3|hZFH|mi-2 autoantigen 240 kDa protein|zinc finger helicase|zinc-finger helicase (Snf2-like)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CHD3, check out the CHD3 Infographic

CHD3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CHD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Mi2- alpha Monoclonal Antibody

Question

I have attached the WB image, lot number and protocol we used for thymus using anti-Mi2- alpha Monoclonal antibody A03200. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-11

Question

We are currently using anti-Mi2- alpha Monoclonal antibody A03200 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says bovine, human, mouse, pig, rat. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

The anti-Mi2- alpha Monoclonal antibody (A03200) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-26

Question

Is there a BSA free version of anti-Mi2- alpha Monoclonal antibody A03200 available?

Verified Customer

Verified customer

Asked: 2017-06-19

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Mi2- alpha Monoclonal antibody A03200 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-06-19

Order DetailsPrice
A03200

100uL

$399

Free Secondary Antibody

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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