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MINDY4 antibody (A32449-1) performs well in ELISA, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of MINDY4 using anti-MINDY4 antibody (A32449-1).
MINDY4 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MINDY4 Antibody (A32449-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
MINDY4 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MINDY4 Antibody (A32449-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-MINDY4 Antibody Picoband®
MINDY4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
MINDY4 antibody was positively detected by WB in:
human K562 whole cell, human A549 whole cell, human A431 whole cell, rat testis tissue, rat PC-12 whole cell, mouse testis tissue, mouse RAW2647 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MINDY4 antibody was positively detected by IHC in:
human thyroid papillary carcinoma tissue, human thyroid papillary carcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-MINDY4 Antibody Picoband®
View all MINDY4 Antibodies
View all MINDY4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human MINDY4 recombinant protein (Position: E414-Q685). Human MINDY4 shares 87.5% amino acid (aa) sequence identity with mouse MINDY4.
Cross-Reactivity
No cross reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
72 kDa
Calculated Molecular Weight
84.4 kDa
Background of MINDY4
MINDY lysine 48 deubiquitinase 4, also known as MINDY4, is a human gene. MINDY4 (MINDY Lysine 48 Deubiquitinase 4, also designated AQP1, AQP-1, CHIP28, C7orf67, and FAM188B) is a protein coding gene located on human chromosome 7p14.3. The human MINDY4 gene encodes a 757 amino acids protein containing 84372 Da. The MINDY4 protein is broadly expressed in testis, lung and other tissues. The MINDY4 gene is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, and frog. 330 organisms have orthologs with human gene MINDY4. MINDY4B is an important paralog of this MINDY4 gene.
Cite This Product
Anti-MINDY4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32449-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Dog
91%
High Homology
Sheep
89%
Moderate Homology
Rat
88%
Moderate Homology
Rabbit
88%
Moderate Homology
Mouse
88%
Moderate Homology
Cow
88%
Moderate Homology
Zebrafish
65%
Low homology
Drosophila
30%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MINDY4 antibody
Immunohistochemistry (IHC) protocol for MINDY4 antibody
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Product Promise
We promise Anti-MINDY4 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A32449-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026