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Product Info Summary
| SKU: | A32449-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-MINDY4 Antibody Picoband®
SKU/Catalog Number
A32449-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-MINDY4 Antibody Picoband® catalog # A32449-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MINDY4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32449-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human MINDY4 recombinant protein (Position: E414-Q685). Human MINDY4 shares 87.5% amino acid (aa) sequence identity with mouse MINDY4.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A32449-1 is reactive to MINDY4 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
84.4 kDa
Background of MINDY4
MINDY lysine 48 deubiquitinase 4, also known as MINDY4, is a human gene. MINDY4 (MINDY Lysine 48 Deubiquitinase 4, also designated AQP1, AQP-1, CHIP28, C7orf67, and FAM188B) is a protein coding gene located on human chromosome 7p14.3. The human MINDY4 gene encodes a 757 amino acids protein containing 84372 Da. The MINDY4 protein is broadly expressed in testis, lung and other tissues. The MINDY4 gene is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, and frog. 330 organisms have orthologs with human gene MINDY4. MINDY4B is an important paralog of this MINDY4 gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A32449-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human K562 whole cell, human A549 whole cell, human A431 whole cell, rat testis tissue, rat PC-12 whole cell, mouse testis tissue, mouse RAW2647 whole cell
IHC: human thyroid papillary carcinoma tissue, human thyroid papillary carcinoma tissue
Validation Images & Assay Conditions
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Western blot analysis of MINDY4 using anti-MINDY4 antibody (A32449-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse testis tissue lysates,
Lane 7: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MINDY4 antigen affinity purified polyclonal antibody (Catalog # A32449-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MINDY4 at approximately 72 kDa. The expected band size for MINDY4 is at 84 kDa.
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IHC analysis of MINDY4 using anti-MINDY4 antibody (A32449-1).
MINDY4 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MINDY4 Antibody (A32449-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of MINDY4 using anti-MINDY4 antibody (A32449-1).
MINDY4 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MINDY4 Antibody (A32449-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-MINDY4 Antibody Picoband® (A32449-1)
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