Product Info Summary
SKU: | A05928-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MIRO1/RHOT1 Antibody Picoband™
SKU/Catalog Number
A05928-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MIRO1/RHOT1 Antibody Picoband™ catalog # A05928-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MIRO1/RHOT1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05928-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MIRO1/RHOT1 recombinant protein (Position: Q63-A596).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05928-1 is reactive to RHOT1 in Human, Mouse, Rat
Applications
A05928-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
71 kDa
Calculated molecular weight
70.784kDa
Background of RHOT1
Mitochondrial Rho GTPase 1 (MIRO1) is an enzyme that in humans is encoded by the RHOT1 gene on chromosome 17. The Ras homolog family member T1 (RHOT) is an atypical Rho Ca2+-binding GTPase that localizes to the mitochondria. RHOT1, the related protein RHOT2, the adaptor protein Milton, and the PTEN induced putative kinase 1 (PINK1), form a complex that is involved in axonal transport of mitochondria. Both PINK1 and Parkin target RHOT1 for phosphorylation and degradation, causing the arrest of mitochondrial motility.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MIRO1/RHOT1 using anti-MIRO1/RHOT1 antibody (A05928-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIRO1/RHOT1 antigen affinity purified polyclonal antibody (Catalog # A05928-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIRO1/RHOT1 at approximately 71 kDa. The expected band size for MIRO1/RHOT1 is at 71 kDa.
Click image to see more details
Figure 2. IHC analysis of MIRO1/RHOT1 using anti-MIRO1/RHOT1 antibody (A05928-1).
MIRO1/RHOT1 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MIRO1/RHOT1 Antibody (A05928-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of MIRO1/RHOT1 using anti-MIRO1/RHOT1 antibody (A05928-1).
MIRO1/RHOT1 was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MIRO1/RHOT1 Antibody (A05928-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of MIRO1/RHOT1 using anti-MIRO1/RHOT1 antibody (A05928-1).
MIRO1/RHOT1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MIRO1/RHOT1 Antibody (A05928-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of MIRO1/RHOT1 using anti-MIRO1/RHOT1 antibody (A05928-1).
MIRO1/RHOT1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MIRO1/RHOT1 Antibody (A05928-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IF analysis of MIRO1/RHOT1 using anti-MIRO1/RHOT1 antibody (A05928-1).
MIRO1/RHOT1 was detected in an immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MIRO1/RHOT1 Antibody (A05928-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For RHOT1 (Source: Uniprot.org, NCBI)
Gene Name
RHOT1
Full Name
Mitochondrial Rho GTPase 1
Weight
70.784kDa
Superfamily
mitochondrial Rho GTPase family
Alternative Names
ARHT1; EC 3.6.5; EC 3.6.5.-; FLJ11040; FLJ12633; hMiro-1; Miro1; MIRO-1mitochondrial Rho GTPase 1; mitochondrial Rho 1; rac-GTP binding protein-like protein; Rac-GTP-binding protein-like protein; Ras homolog gene family member T1; ras homolog gene family, member T1; Rhot1 RHOT1 ARHT1, MIRO-1, MIRO1 ras homolog family member T1 mitochondrial Rho GTPase 1|mitochondrial Rho (MIRO) GTPase 1|rac-GTP binding protein-like protein|ras homolog gene family, member T1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RHOT1, check out the RHOT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RHOT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MIRO1/RHOT1 Antibody Picoband™ (A05928-1)
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