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- Table of Contents
RHOT1 antibody (A05928) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of RHOT in mouse brain tissue with RHOT antibody at 20 μg/ml.
Designing a Western blot or IHC for RHOT1? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-Mitochondrial Rho GTPase 1 RHOT1 Antibody
RHOT1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
RHOT1 antibody can be used for detection of RHOT1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Mitochondrial Rho GTPase 1 RHOT1 Antibody
View all RHOT1 Antibodies
View all RHOT1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
RHOT1 antibody can be stored at 4°C, stable for up to one year.
Host
Rabbit
Contents
RHOT1 antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RHOT1 antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOT1. The immunogen is located within amino acids 40 - 90 of RHOT1.
Cross-Reactivity
RHOT1 antibody is human, mouse and rat reactive. At least three isoforms of RHOT1 are known to exist; this antibody will detect all three isoforms.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
70.8 kDa
Background of RHOT1
The Ras homolog family member T1 (RHOT) is an atypical Rho Ca2+-binding GTPase that localizes to the mitochondria (1). RHOT1, the related protein RHOT2, the adaptor protein Milton, and the PTEN induced putative kinase 1 (PINK1), form a complex that is involved in axonal transport of mitochondria (2,3). Both PINK1 and Parkin target RHOT1 for phosphorylation and degradation, causing the arrest of mitochondrial motility (4).
Cite This Product
Anti-Mitochondrial Rho GTPase 1 RHOT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05928)
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Product Promise
We promise Anti-Mitochondrial Rho GTPase 1 RHOT1 Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A05928
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 31, 2026