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Product Info Summary
| SKU: | A05928 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Mitochondrial Rho GTPase 1 RHOT1 Antibody
SKU/Catalog Number
A05928
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Mitochondrial Rho GTPase 1 RHOT1 Antibody (Catalog # A05928). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
RHOT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Mitochondrial Rho GTPase 1 RHOT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05928)
Host
Rabbit
Contents
RHOT1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RHOT1 antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOT1. The immunogen is located within amino acids 40 - 90 of RHOT1.
Cross-reactivity
RHOT1 antibody is human, mouse and rat reactive. At least three isoforms of RHOT1 are known to exist; this antibody will detect all three isoforms.
Reactive Species
A05928 is reactive to RHOT1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
70.8 kDa
Background of RHOT1
The Ras homolog family member T1 (RHOT) is an atypical Rho Ca2+-binding GTPase that localizes to the mitochondria (1). RHOT1, the related protein RHOT2, the adaptor protein Milton, and the PTEN induced putative kinase 1 (PINK1), form a complex that is involved in axonal transport of mitochondria (2,3). Both PINK1 and Parkin target RHOT1 for phosphorylation and degradation, causing the arrest of mitochondrial motility (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05928 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RHOT1 antibody can be used for detection of RHOT1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RHOT1 in rat brain tissue lysate with RHOT1 antibody at 1 μg/ml.
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Immunohistochemistry of RHOT in mouse brain tissue with RHOT antibody at 5 μg/ml.
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Immunofluorescence of RHOT in mouse brain tissue with RHOT antibody at 20 μg/ml.
Specific Publications For Anti-Mitochondrial Rho GTPase 1 RHOT1 Antibody (A05928)
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