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Product Info Summary
| SKU: | M00269-3 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-MITF Antibody
SKU/Catalog Number
M00269-3
Size
50 µl
Description
Boster Bio Anti-MITF Antibody (Catalog # M00269-3). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MITF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00269-3)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1607CT834.207.47
Isotype
IgG1,k
Immunogen
This MITF antibody is generated from a mouse immunized with a recombinant protein of human MITF.
Reactive Species
M00269-3 is reactive to MITF in Human, Mouse, Rat
Calculated molecular weight
58.8 kDa
Background of MITF
Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'- CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00269-3 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC: 1:1000
WB: 1:2000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human Melanoma section using Pink1. M00269-3 was diluted at 1:1000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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All lanes : Anti-MITF Antibody at 1:2000 dilution
Lane 1: A431 whole cell lysate
Lane 2: PC-12 whole cell lysate
Lane 3: NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 59 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-MITF Antibody (M00269-3)
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