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Product Info Summary
| SKU: | A10727-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband™
SKU/Catalog Number
A10727-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband™ catalog # A10727-3. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10727-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Mitochondrial dicarboxylate carrier/SLC25A10 recombinant protein (Position: Q37-Q276).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10727-3 is reactive to SLC25A10 in Human, Mouse, Rat
Applications
A10727-3 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
29-31 kDa
Calculated molecular weight
31.282kDa
Background of SLC25A10
The mitochondrial dicarboxylate carrier (DIC) is an integral membrane protein encoded by the SLC25A10 gene in humans that catalyzes the transport of dicarboxylates such as malonate, malate, and succinate across the inner mitochondrial membrane in exchange for phosphate, sulfate, and thiosulfate by a simultaneous antiport mechanism, thus supplying substrates for the Krebs cycle, gluconeogenesis, urea synthesis, fatty acid synthesis, and sulfur metabolism. This gene encodes a member of a family of proteins that translocate small metabolites across the mitochondrial membrane. The encoded protein exchanges dicarboxylates, such as malate and succinate, for phosphate, sulfate, and other small molecules, thereby providing substrates for metabolic processes including the Krebs cycle and fatty acid synthesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse, Rat
Immunofluorescence, 5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antigen affinity purified polyclonal antibody (Catalog # A10727-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitochondrial Dicarboxylate Carrier/SLC25A10 at approximately 29-31 kDa. The expected band size for Mitochondrial Dicarboxylate Carrier/SLC25A10 is at 31 kDa.
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Figure 2. IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3).
Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3).
Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3).
Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3).
Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3).
Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3).
Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human diffuse large B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 8. IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3).
Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 9. IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3).
Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 10. IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3).
Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 11. IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3).
Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human right renal oncocytoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 12. IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3).
Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 13. IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3).
Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 14. IF analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3).
Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For SLC25A10 (Source: Uniprot.org, NCBI)
Gene Name
SLC25A10
Full Name
Mitochondrial dicarboxylate carrier
Weight
31.282kDa
Superfamily
mitochondrial carrier (TC 2.A.29) family
Alternative Names
dicarboxylate ion carrier; DICSolute carrier family 25 member 10; member 10; mitochondrial dicarboxylate carrier; solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter) SLC25A10 DIC, MTDPS19 solute carrier family 25 member 10 mitochondrial dicarboxylate carrier|dicarboxylate ion carrier|solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter), member 10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC25A10, check out the SLC25A10 Infographic
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In this infographic, you will see the following information for SLC25A10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband™ (A10727-3)
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