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Product Info Summary
| SKU: | M03483 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-MKLP1 KIF23 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03483
BM3909 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MKLP1 KIF23 Rabbit Monoclonal Antibody catalog # M03483. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MKLP1 KIF23 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03483)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EG-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MKLP1
Reactive Species
M03483 is reactive to KIF23 in Human, Mouse, Rat
Observed Molecular Weight
98 kDa
Calculated molecular weight
110.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03483 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Positive Control
WB: human Hela whole cell, human SiHa whole cell, human Hacat whole cell lysates, human U251 whole cell, rat brain tissue
Validation Images & Assay Conditions
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Western blot analysis of KIF23 using anti-KIF23 antibody (M03483).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF23 antigen affinity purified monoclonal antibody (Catalog # M03483) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF23 at approximately 98 kDa. The expected band size for KIF23 is at 98 kDa.
Specific Publications For Anti-MKLP1 KIF23 Rabbit Monoclonal Antibody (M03483)
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Customer Q&As
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5 Customer Q&As for Anti-MKLP1 KIF23 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-MKLP1 Rabbit Monoclonal antibody (M03483)?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
We do provide the blocking peptide for product anti-MKLP1 Rabbit Monoclonal antibody (M03483). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-02
Question
We are currently using anti-MKLP1 Rabbit Monoclonal antibody M03483 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
The anti-MKLP1 Rabbit Monoclonal antibody (M03483) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-13
Question
Is there a BSA free version of anti-MKLP1 Rabbit Monoclonal antibody M03483 available?
W. Zhang
Verified customer
Asked: 2018-06-06
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MKLP1 Rabbit Monoclonal antibody M03483 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-06
Question
Is this M03483 anti-MKLP1 Rabbit Monoclonal antibody reactive to the isotypes of KIF23?
N. Jackson
Verified customer
Asked: 2015-09-23
Answer
The immunogen of M03483 anti-MKLP1 Rabbit Monoclonal antibody is A synthesized peptide derived from human MKLP1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-09-23
Question
Will anti-MKLP1 Rabbit Monoclonal antibody M03483 work for IF with lymph uterus?
G. Parker
Verified customer
Asked: 2015-05-21
Answer
According to the expression profile of lymph uterus, KIF23 is highly expressed in lymph uterus. So, it is likely that anti-MKLP1 Rabbit Monoclonal antibody M03483 will work for IF with lymph uterus.
Boster Scientific Support
Answered: 2015-05-21
