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Product Info Summary
| SKU: | A05807 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-MKRN3 Antibody
SKU/Catalog Number
A05807
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MKRN3 Antibody (Catalog # A05807). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
MKRN3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-MKRN3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05807)
Host
Rabbit
Contents
MKRN3 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MKRN3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human MKRN3. The immunogen is located within amino acids 400 - 450 of MKRN3.
Cross-reactivity
MKRN3 antibody is human specific. MKRN3 antibody is predicted to not cross-react with other members of the MKRN protein family.
Reactive Species
A05807 is reactive to MKRN3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Background of MKRN3
The Makorin ring finger 3 (MKRN3) protein contains a RING (C3HC4) zinc finger motif and several C3H zinc finger motifs. The MKRN3 gene is intronless and imprinted, with expression only from the paternal allele. Disruption of the imprinting at this locus may contribute to Prader-Willi syndrome (1), but a deletion of the gene does not (2). A deficiency of MKRN3 has been shown to cause central precocious puberty in humans (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05807 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MKRN3 antibody can be used for detection of MKRN3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MKRN3 in human spleen tissue lysate with MKRN3 antibody at (A)1 and (B) 2 μg/ml.
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Immunofluorescence of MKRN3 in human spleen tissue with MKRN3 antibody at 20 μg/mL.
Specific Publications For Anti-MKRN3 Antibody (A05807)
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