Click image to see more details
Product Info Summary
| SKU: | A06772 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Myeloid leukemia factor 1 MLF1 Antibody
View all Myeloid leukemia factor 1 Antibodies
SKU/Catalog Number
A06772
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Myeloid leukemia factor 1 MLF1 Antibody (Catalog # A06772). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myeloid leukemia factor 1 MLF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06772)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MLF1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06772 is reactive to MLF1 in Human, Mouse
Applications
A06772 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
30.627kDa
Background of Myeloid leukemia factor 1
Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
Yoneda-Kato N., Oncogene 12:265-275(1996) [PubMed: 8570204].
Hanissian S.H., Oncogene 23:3700-3707(2004) [PubMed: 15116101].
Yoneda-Kato N., EMBO J. 24:1739-1749(2005) [PubMed: 15861129].
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HUVEC cells, using MLF1 antibody A06772.
Protein Target Info & Infographic
Gene/Protein Information For MLF1 (Source: Uniprot.org, NCBI)
Gene Name
MLF1
Full Name
Myeloid leukemia factor 1
Weight
30.627kDa
Superfamily
MLF family
Alternative Names
Myelodysplasia-myeloid leukemia factor 1; myeloid leukemia factor 1 MLF1 myeloid leukemia factor 1 myeloid leukemia factor 1|myelodysplasia-myeloid leukemia factor 1|testis tissue sperm-binding protein Li 49e
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MLF1, check out the MLF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MLF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myeloid leukemia factor 1 MLF1 Antibody (A06772)
Hello CJ!
No publications found for A06772
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Myeloid leukemia factor 1 MLF1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Myeloid leukemia factor 1 MLF1 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-Myeloid leukemia factor 1 MLF1 Antibody
Question
Does anti-MLF1 antibody A06772 work for WB with brain skeletal muscle?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
According to the expression profile of brain skeletal muscle, MLF1 is highly expressed in brain skeletal muscle. So, it is likely that anti-MLF1 antibody A06772 will work for WB with brain skeletal muscle.
Boster Scientific Support
Answered: 2020-04-28
Question
I was wanting to use your anti-MLF1 antibody for WB for mouse brain skeletal muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain skeletal muscle identification?
Verified Customer
Verified customer
Asked: 2018-01-16
Answer
As indicated on the product datasheet, A06772 anti-MLF1 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain skeletal muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-16
Question
We are currently using anti-MLF1 antibody A06772 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
The anti-MLF1 antibody (A06772) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-09
