Click image to see more details
Product Info Summary
| SKU: | A05171-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-MMP-19 Antibody
SKU/Catalog Number
A05171-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MMP-19 Antibody catalog # A05171-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP-19 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05171-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
4A11A6G11
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MMP-19. AA range:11-60
Reactive Species
A05171-1 is reactive to MMP19 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
57.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05171-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MMP-19 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of various cells using MMP-19 Polyclonal Antibody diluted at 1:500
Specific Publications For Anti-MMP-19 Antibody (A05171-1)
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Customer Q&As
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4 Customer Q&As for Anti-MMP-19 Antibody
Question
Does A05171-1 anti-MMP-19 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
As indicated on the product datasheet, A05171-1 anti-MMP-19 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-08
Question
Is this A05171-1 anti-MMP-19 antibody reactive to the isotypes of MMP19?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
The immunogen of A05171-1 anti-MMP-19 antibody is Synthesized peptide derived from the N-terminal region of human MMP-19. at AA rangle: 30-110. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-24
Question
Please see the WB image, lot number and protocol we used for rheumatoid arthritic synovial fluid using anti-MMP-19 antibody A05171-1. Please let me know if you require anything else.
J. Wu
Verified customer
Asked: 2018-11-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-16
Question
We are currently using anti-MMP-19 antibody A05171-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on zebrafish tissues as well?
B. Johnson
Verified customer
Asked: 2013-12-31
Answer
The anti-MMP-19 antibody (A05171-1) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-12-31
