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Product Info Summary
| SKU: | A06693 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MMS21 NSMCE2 Antibody
SKU/Catalog Number
A06693
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MMS21 NSMCE2 Antibody (Catalog # A06693). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
MMS21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-MMS21 NSMCE2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06693)
Host
Rabbit
Contents
MMS21 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MMS21 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human MMS21. The immunogen is located within amino acids 170 - 220 of MMS21.
Reactive Species
A06693 is reactive to NSMCE2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
27.9 kDa
Background of NSMCE2
MMS21, also known as NSE2, is a SUMO ligase that in combination with the SMC5/6 complex is required for the prevention of DNA damage induced apoptosis by facilitating DNA repair in human cells. MMS21-dependent sumoylation is integral and important to the cohesion mechanism and mitotic progression; this function appears to be independent of SMC6. MMS21 mediates SUMO attachment to various proteins such as SMC6L1 and TRAX, and possibly the cohesin components RAD21 and STAG2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06693 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MMS21 antibody can be used for detection of MMS21 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MMS21 in 293 cell lysate with MMS21 antibody at (A) 0.5 and (B) 1 μg/mL.
Specific Publications For Anti-MMS21 NSMCE2 Antibody (A06693)
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