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Product Info Summary
| SKU: | A16079-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-MOB3A/B/C Antibody Picoband®
SKU/Catalog Number
A16079-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MOB3A/B/C Antibody Picoband® catalog # A16079-3. Tested in WB,FCM,ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MOB3A/B/C Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16079-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human MOB3A/B/C recombinant protein (Position: E30-H217). Human MOB3A/B/C shares 93.6% amino acid (aa) sequence identity with mouse MOB3A/B/C.
Reactive Species
A16079-3 is reactive to MOB3A/B/C in Human, Mouse, Rat
Observed Molecular Weight
25 kDa
Calculated molecular weight
25.5 kDa
Background of MOB3A/B/C
MOB3A encodes a protein that is part of the MOB (Mps One Binder) family. This protein is involved in regulating cell cycle progression, particularly in the transition from G1 to S phase. MOB3A interacts with various proteins, influencing cellular processes such as proliferation and signaling pathways. Its role in cell cycle regulation and potential involvement in other cellular functions makes it a significant area of study.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16079-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Human, Mouse, Rat |
| Flow Cytometry (Fixed) | 1-3 μg/1x106 cells | Human |
| ELISA | 0.1-0.5 μg/ml | - |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of MOB3A/B/C using anti-MOB3A/B/C antibody (A16079-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: rat thymus tissue lysates,
Lane 5: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOB3A/B/C antigen affinity purified polyclonal antibody (Catalog # A16079-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MOB3A/B/C at approximately 25 kDa. The expected band size for MOB3A/B/C is at 25 kDa.
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Flow Cytometry analysis of Raji cells using anti-MOB3A/B/C antibody (A16079-3).
Overlay histogram showing Raji cells stained with A16079-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MOB3A/B/C Antibody (A16079-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-MOB3A/B/C Antibody Picoband® (A16079-3)
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