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Product Info Summary
| SKU: | A12301-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-Mouse Csnk1g3 Antibody (C-term)
SKU/Catalog Number
A12301-1
Size
80 µl
Description
Boster Bio Anti-Mouse Csnk1g3 Antibody (C-term) (Catalog # A12301-1). Tested in Flow Cytometry, IHC-P, WB application(s). This antibody reacts with Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-Mouse Csnk1g3 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12301-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Mouse Csnk1g3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 333-361 amino acids from the C-terminal region of mouse Csnk1g3.
Reactive Species
A12301-1 is reactive to Csnk1g3 in Mouse
Calculated molecular weight
48.9 kDa
Background of Csnk1g3
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12301-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysates from mouse kidney and liver tissue lysate (from left to right), using Mouse Csnk1g3 Antibody (C-term). A12301-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Mouse Csnk1g3 Antibody (C-term) (A12301-1)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Csnk1g3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Immunohistochemical analysis of paraffin-embedded M. liver section using Mouse Csnk1g3 Antibody (C-term). A12301-1 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of NIH/3T3 cells using Mouse Csnk1g3 Antibody (C-term)(green, Cat#A12301-1) compared to an isotype control of rabbit IgG(blue). A12301-1 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Specific Publications For Anti-Mouse Csnk1g3 Antibody (C-term) (A12301-1)
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