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Product Info Summary
| SKU: | A05192 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-Mouse Ctr9 Antibody (C-term)
SKU/Catalog Number
A05192
Size
80 µl
Description
Boster Bio Anti-Mouse Ctr9 Antibody (C-term) (Catalog # A05192). Tested in IF, IHC-P, WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-Mouse Ctr9 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05192)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Mouse Ctr9 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1022-1056 amino acids from the C-terminal region of Mouse Ctr9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05192 is reactive to Ctr9 in Human, Mouse
Reconstitution
Calculated molecular weight
133408 Da
Background of CTR9
Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys- 4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Required for mono- and trimethylation on histone H3 'Lys- 4' (H3K4me3) and dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription (By similarity). Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of the SET1 complex. Involved in transcriptional regulation of IL6-responsive genes and in JAK-STAT pathway; may regulate DNA-association of STAT3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05192 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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Fluorescent image of A431 cells stained with Mouse Ctr9 Antibody (C-term). A05192 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Western blot analysis of lysates from Jurkat, HT-29, mouse BA/F3, mouse F9 cell line, mouse cerebellum, mouse testis tissue (from left to right), using Mouse Ctr9 Antibody (C-term) (Cat. #A05192). A05192 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Immunohistochemical analysis of paraffin-embedded M. testis section using Mouse Ctr9 Antibody (C-term) (Cat#A05192). A05192 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded M. colon section using Mouse Ctr9 Antibody (C-term) (Cat#A05192). A05192 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Protein Target Info & Infographic
Gene/Protein Information For Ctr9 (Source: Uniprot.org, NCBI)
Gene Name
Ctr9
Full Name
RNA polymerase-associated protein CTR9 homolog
Weight
133408 Da
Alternative Names
RNA polymerase-associated protein CTR9 homolog, SH2 domain-binding protein 1, Tetratricopeptide repeat-containing, SH2-binding phosphoprotein of 150 kDa, TPR-containing, SH2-binding phosphoprotein of 150 kDa, p150TSP, Ctr9, Kiaa0155, Sh2bp1 CTR9 SH2BP1, TSBP, p150, p150TSP CTR9 homolog, Paf1/RNA polymerase II complex component RNA polymerase-associated protein CTR9 homolog|Ctr9, Paf1/RNA polymerase II complex component, homolog|SH2 domain binding protein 1 (tetratricopeptide repeat containing)|TPR-containing, SH2-binding phosphoprotein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ctr9, check out the Ctr9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ctr9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mouse Ctr9 Antibody (C-term) (A05192)
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