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- Table of Contents
Med12 antibody (M00828) performs well in IHC-P, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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M00828 staining Mouse Med12 in mouse stomach sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Anti-Mouse Med12 Antibody (C-term)
Med12 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1:2000
IHC-P: 1:25
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Product Information
Product Name
Anti-Mouse Med12 Antibody (C-term)
View all MED12 Antibodies
View all MED12 Antibodies
Uniprot
Gene ID
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Mouse Med12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1991-2025 amino acids from the C-terminal region of Mouse Med12.
Calculated Molecular Weight
244.6 kDa
Background of Med12
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity).
Cite This Product
Anti-Mouse Med12 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00828)
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Product Promise
We promise Anti-Mouse Med12 Antibody (C-term) performs excellently in relevant applications for IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00828
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026