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Product Info Summary
| SKU: | M00828 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-Mouse Med12 Antibody (C-term)
SKU/Catalog Number
M00828
Size
80 µl
Description
Boster Bio Anti-Mouse Med12 Antibody (C-term) (Catalog # M00828). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-Mouse Med12 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00828)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Mouse Med12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1991-2025 amino acids from the C-terminal region of Mouse Med12.
Reactive Species
M00828 is reactive to Med12 in Human, Mouse
Calculated molecular weight
244.6 kDa
Background of Med12
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00828 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-Med12 Antibody (C-term) at 1:2000 dilution
Lane 1: Hela whole cell lysates
Lane 2: C2C12 whole cell lysates
Lane 3: Jurkat whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 245 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-Med12 Antibody (C-term)at 1:1000 dilution + NIH/3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 245 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Anti-Med12 Antibody (C-term)at 1:1000 dilution + mouse spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 245 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
M00828 staining Mouse Med12 in mouse stomach sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-Mouse Med12 Antibody (C-term) (M00828)
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