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Product Info Summary
| SKU: | M05571 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Mouse Noto Antibody (C-term)
SKU/Catalog Number
M05571
Size
80 µl
Description
Boster Bio Anti-Mouse Noto Antibody (C-term) (Catalog # M05571). Tested in WB application(s). This antibody reacts with Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-Mouse Noto Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05571)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Mouse Noto antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 200-234 amino acids from the C-terminal region of Mouse Noto.
Reactive Species
M05571 is reactive to Noto in Mouse
Calculated molecular weight
26.3 kDa
Background of Noto
Transcription regulator acting downstream of both FOXA2 and T during notochord development. Required for node morphogenesis. Is essential for cilia formation in the posterior notochord (PNC) and for left-right patterning; acts upstream of FOXJ1 and RFX3 in this process and is required for the expression of various components important for axonemal assembly and function. Plays a role in regulating axial versus paraxial cell fate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05571 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
Validation Images & Assay Conditions
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Western blot analysis of lysate from mouse liver tissue lysate, using Noto Antibody (C-term). M05571 was diluted at 1:2000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Western blot analysis of lysates from mouse liver, mouse pancreas tissue lysate (from left to right), using Noto Antibody (C-term). M05571 was diluted at 1:4000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Specific Publications For Anti-Mouse Noto Antibody (C-term) (M05571)
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