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Product Info Summary
| SKU: | A06352-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-Mouse Srms Antibody (Center)
SKU/Catalog Number
A06352-1
Size
80 µl
Description
Boster Bio Anti-Mouse Srms Antibody (Center) (Catalog # A06352-1). Tested in WB, IF application(s). This antibody reacts with Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-Mouse Srms Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06352-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Mouse Srms antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 113-147 amino acids from the Central region of Mouse Srms.
Reactive Species
A06352-1 is reactive to Srms in Mouse
Calculated molecular weight
55.8 kDa
Background of Srms
Non-receptor tyrosine-protein kinase which phosphorylates DOK1 on tyrosine residues. May be involved in proliferation or differentiation of keratinocytes in the skin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06352-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse skin tissue labeling Mouse Srms with A06352-1 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Confocal image showing cytoplasm staining on mouse skin tissue. The nuclear counter stain is DAPI (blue).
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Western blot analysis of lysates from mouse liver, mouse spleen tissue lysate (from left to right), using Srms Antibody (Center). A06352-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Anti-Srms Antibody (C-term) at 1:2000 dilution + mouse liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 56 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-Mouse Srms Antibody (Center) (A06352-1)
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