Anti-(Mouse) Tdgf1 Antibody (N-term)

Product Name

Anti-(Mouse) Tdgf1 Antibody (N-term)

View all CRIPTO Antibodies

SKU/Catalog Number

A03105

Size

80 µl

Description

Boster Bio Anti-(Mouse) Tdgf1 Antibody (N-term) (Catalog # A03105). Tested in WB, Flow Cytometry application(s). This antibody reacts with Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-(Mouse) Tdgf1 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03105)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This Mouse Tdgf1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 37-70 amino acids from the N-terminal region of mouse Tdgf1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03105 is reactive to TDGF1 in Mouse

Applications

A03105 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

21.169kDa

Background of CRIPTO

Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
FC: 1:25

Validation Images & Assay Conditions

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Western blot analysis of lysate from mouse F9 cell line, using (Mouse) Tdgf1 Antibody (N-term). A03105 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

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Overlay histogram showing F9 cells stained with A03105 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A03105, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Gene/Protein Information For TDGF1 (Source: Uniprot.org, NCBI)

Alternative Names

CFC-2; CR; Cripto; Cripto-1 growth factor; Cripto-1; CRIPTOCRGF; Epidermal growth factor-like cripto protein CR1; TDGF1; TDGF-1; teratocarcinoma-derived growth factor 1 TDGF1 CR, CR-1, CRGF, CRIPTO teratocarcinoma-derived growth factor 1 teratocarcinoma-derived growth factor 1|cripto-1 growth factor|epidermal growth factor-like cripto protein CR1

For more info on TDGF1, check out the TDGF1 Infographic

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In this infographic, you will see the following information for TDGF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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