Anti-MPPE1 Antibody Picoband™

Product Name

Anti-MPPE1 Antibody Picoband™

View all MPPE1 Antibodies

SKU/Catalog Number

A12491-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-MPPE1 Antibody Picoband™ catalog # A12491-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-MPPE1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12491-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MPPE1 recombinant protein (Position: Y43-R329).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A12491-1 is reactive to MPPE1 in Human, Mouse, Rat

Applications

A12491-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

45 kDa

Calculated molecular weight

45.141kDa

Background of MPPE1

MPPE1 encodes a metallophosphoesterase protein that is widely brain expressed and is a member of the calcineurin-like phosphoesterase superfamily. Phosphoesterases are involved in a variety of diverse biochemical reactions, including protein phosphorylation-dephosphorylation processes that modulate functional properties of proteins. The MPPE1 gene is located on chromosome 18p11.21 and is flanked by the G protein Golf alpha (GNAL) gene and the myo-inositol monophosphatase gene (IMPA2). Variation in the MPPE1 gene might lead to an altered enzyme with downstream effects on protein phosphorylation involved in cellular signaling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of MPPE1 using anti-MPPE1 antibody (A12491-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPPE1 antigen affinity purified polyclonal antibody (Catalog # A12491-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPPE1 at approximately 45 kDa. The expected band size for MPPE1 is at 45 kDa.

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Figure 2. Flow Cytometry analysis of RT4 cells using anti-MPPE1 antibody (A12491-1).
Overlay histogram showing RT4 cells stained with A12491-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MPPE1 Antibody (A12491-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For MPPE1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.1; metallo phosphoesterase; metallophosphoesterase 1; PGAP5; Post-GPI attachment to proteins factor 5 MPPE1 Cdc1, PGAP5 metallophosphoesterase 1 metallophosphoesterase 1|metallo phosphoesterase|post-GPI attachment to proteins 5|post-GPI attachment to proteins factor 5

For more info on MPPE1, check out the MPPE1 Infographic

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In this infographic, you will see the following information for MPPE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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