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Product Info Summary
| SKU: | A16446 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MRGX4 MRGPRX4 Antibody
SKU/Catalog Number
A16446
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MRGX4 MRGPRX4 Antibody catalog # A16446. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRGX4 MRGPRX4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16446)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MRGX4. AA range:271-320
Reactive Species
A16446 is reactive to MRGPRX4 in Human
Calculated molecular weight
36.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16446 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Western blot analysis of lysates from HUVEC and COLO cells, using MRGX4 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Immunofluorescence analysis of A549 cells, using MRGX4 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-MRGX4 MRGPRX4 Antibody (A16446)
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Customer Q&As
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4 Customer Q&As for Anti-MRGX4 MRGPRX4 Antibody
Question
Can you help my question with product A16446, anti-MRGX4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A16446 anti-MRGX4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-18
Question
Is this A16446 anti-MRGX4 antibody reactive to the isotypes of MRGPRX4?
J. Huang
Verified customer
Asked: 2015-12-23
Answer
The immunogen of A16446 anti-MRGX4 antibody is Synthesized peptide derived from the C-terminal region of human MRGX4. at AA rangle: 240-320. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-12-23
Question
See attached the WB image, lot number and protocol we used for middle temporal gyrus using anti-MRGX4 antibody A16446. Please let me know if you require anything else.
S. Parker
Verified customer
Asked: 2014-11-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-11-05
Question
I am interested in to test anti-MRGX4 antibody A16446 on human middle temporal gyrus for research purposes, then I may be interested in using anti-MRGX4 antibody A16446 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Walker
Verified customer
Asked: 2014-02-28
Answer
The products we sell, including anti-MRGX4 antibody A16446, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-02-28
