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Product Info Summary
| SKU: | A15741 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-MRP-L21 Antibody
SKU/Catalog Number
A15741
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MRP-L21 Antibody catalog # A15741. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP-L21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15741)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPL21. AA range:131-180
Reactive Species
A15741 is reactive to MRPL21 in Human, Mouse
Calculated molecular weight
22.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15741 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MRPL21 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of various cells using MRP-L21 Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysates from COLO cells, using MRPL21 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HeLa cells using MRPL21 antibody.
Specific Publications For Anti-MRP-L21 Antibody (A15741)
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Customer Q&As
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6 Customer Q&As for Anti-MRP-L21 Antibody
Question
Do you have a BSA free version of anti-MRP-L21 antibody A15741 available?
Verified Customer
Verified customer
Asked: 2018-09-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MRP-L21 antibody A15741 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-20
Question
We are currently using anti-MRP-L21 antibody A15741 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
The anti-MRP-L21 antibody (A15741) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-05
Question
We want to test anti-MRP-L21 antibody A15741 on mouse melanoma for research purposes, then I may be interested in using anti-MRP-L21 antibody A15741 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-13
Answer
The products we sell, including anti-MRP-L21 antibody A15741, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-13
Question
I have a question about product A15741, anti-MRP-L21 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A15741 anti-MRP-L21 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-08
Question
Is a blocking peptide available for product anti-MRP-L21 antibody (A15741)?
P. Banerjee
Verified customer
Asked: 2015-07-03
Answer
We do provide the blocking peptide for product anti-MRP-L21 antibody (A15741). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-07-03
Question
Is this A15741 anti-MRP-L21 antibody reactive to the isotypes of MRPL21?
R. Anderson
Verified customer
Asked: 2015-04-17
Answer
The immunogen of A15741 anti-MRP-L21 antibody is Synthesized peptide derived from the Internal region of human MRP-L21. at AA rangle: 100-180. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-04-17
