Click image to see more details
Product Info Summary
| SKU: | A14430-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MRP-L4 Antibody
View all mitochondrial ribosomal protein L4 Antibodies
SKU/Catalog Number
A14430-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MRP-L4 Antibody catalog # A14430-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP-L4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14430-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human MRP-L4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14430-1 is reactive to MRPL4 in Human, Mouse
Applications
A14430-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
34.919kDa
Background of mitochondrial ribosomal protein L4
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms.
Suzuki T., J. Biol. Chem. 276:21724-21736(2001).
Lai C.-H., Genome Res. 10:703-713(2000).
Grimwood J., Nature 428:529-535(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of MRPL4 using Anti-MRP-L4 Antibody (A14430-1).
Immunohistochemical analysis of paraffin-embedded human lung cancer. Antibody was diluted at 1:100 (4°C
Click image to see more details
Figure 1. Western blotting validation for Anti-MRP-L4 Antibody A14430-1
Western Blot (WB) analysis of specific cells using MRP-L4 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For MRPL4 (Source: Uniprot.org, NCBI)
Gene Name
MRPL4
Full Name
39S ribosomal protein L4, mitochondrial
Weight
34.919kDa
Superfamily
universal ribosomal protein uL4 family
Alternative Names
CGI-28; L4mt; MGC16367; MGC2681; mitochondrial ribosomal protein L4,39S ribosomal protein L4, mitochondrial; MRP-L4 MRPL4 CGI-28, L4mt mitochondrial ribosomal protein L4 39S ribosomal protein L4, mitochondrial|MRP-L4|mitochondrial large ribosomal subunit protein uL4m
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRPL4, check out the MRPL4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRPL4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRP-L4 Antibody (A14430-1)
Hello CJ!
No publications found for A14430-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MRP-L4 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-MRP-L4 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
