Product Info Summary
SKU: | A14514S21-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-MRP-S21 Antibody
SKU/Catalog Number
A14514S21-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MRP-S21 Antibody catalog # A14514S21-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP-S21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14514S21-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human MRP-S21
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14514S21-1 is reactive to MRPS21 in Human, Monkey, Mouse, Rat
Applications
A14514S21-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
10.689kDa
Background of MRPS21
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5' UTR; both transcripts encode the same protein.
Suzuki T., J. Biol. Chem. 276:33181-33195(2001).
Huang C., Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of MRPS21 using Anti-MRP-S21 Antibody (A14514S21-1).
Immunohistochemical analysis of paraffin-embedded Human pancreas. Antibody was diluted at 1:100 (4°CClick image to see more details
Figure 1. Western blotting validation for Anti-MRP-S21 Antibody A14514S21-1
Western Blot (WB) analysis of specific cells using MRP-S21 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For MRPS21 (Source: Uniprot.org, NCBI)
Gene Name
MRPS21
Full Name
28S ribosomal protein S21, mitochondrial
Weight
10.689kDa
Superfamily
bacterial ribosomal protein bS21 family
Alternative Names
28S ribosomal protein S21, mitochondrial; mitochondrial 28S ribosomal protein S21; mitochondrial ribosomal protein S21; MRP-S21; RPMS21; S21mt MRPS21 MDS016, MRP-S21, RPMS21 mitochondrial ribosomal protein S21 28S ribosomal protein S21, mitochondrial|S21mt|mitochondrial 28S ribosomal protein S21|mitochondrial small ribosomal subunit protein bS21m
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRPS21, check out the MRPS21 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRPS21: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRP-S21 Antibody (A14514S21-1)
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No publications found for A14514S21-1
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