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Product Info Summary
| SKU: | M00413-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-MRP8 S100A8 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00413-1
BM4221 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MRP8 S100A8 Rabbit Monoclonal Antibody catalog # M00413-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP8 S100A8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00413-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CFC-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MRP8
Reactive Species
M00413-1 is reactive to S100A8 in Human, Mouse
Observed Molecular Weight
11 kDa
Calculated molecular weight
10.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00413-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:30
Positive Control
WB: HL-60 cell
IHC: human breast cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of MRP8 expression in HL-60 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using MRP8 Antibody.
Specific Publications For Anti-MRP8 S100A8 Rabbit Monoclonal Antibody (M00413-1)
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Customer Q&As
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4 Customer Q&As for Anti-MRP8 S100A8 Rabbit Monoclonal Antibody
Question
Our lab used your anti-MRP8 Rabbit Monoclonal antibody for IHC on pharyngeal mucosa in a previous project. I am using mouse, and We want to use the antibody for IP next. I was wanting to use examining pharyngeal mucosa as well as ascites in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
I looked at the website and datasheets of our anti-MRP8 Rabbit Monoclonal antibody and it appears that M00413-1 has been tested on mouse in both IHC and IP. Thus M00413-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-20
Question
We are currently using anti-MRP8 Rabbit Monoclonal antibody M00413-1 for mouse tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
The anti-MRP8 Rabbit Monoclonal antibody (M00413-1) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-31
Question
Our team were satisfied with the WB result of your anti-MRP8 Rabbit Monoclonal antibody. However we have seen positive staining in ascites secreted. cytoplasm. cytoplasm, using this antibody. Is that expected? Could you tell me where is S100A8 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
Based on literature, ascites does express S100A8. Generally S100A8 expresses in secreted. cytoplasm. cytoplasm,. Regarding which tissues have S100A8 expression, here are a few articles citing expression in various tissues:
Ascites, Pubmed ID: 7695842
Keratinocyte, Pubmed ID: 1286667
Liver, Pubmed ID: 24275569
Neutrophil, Pubmed ID: 1326551
Skeletal muscle, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-09-16
Question
We have been able to see staining in human ascites. What should we do? Is anti-MRP8 Rabbit Monoclonal antibody supposed to stain ascites positively?
Verified Customer
Verified customer
Asked: 2017-08-22
Answer
From literature ascites does express S100A8. From Uniprot.org, S100A8 is expressed in pharyngeal mucosa, tongue, skeletal muscle, neutrophil, ascites, keratinocyte, liver, among other tissues. Regarding which tissues have S100A8 expression, here are a few articles citing expression in various tissues:
Ascites, Pubmed ID: 7695842
Keratinocyte, Pubmed ID: 1286667
Liver, Pubmed ID: 24275569
Neutrophil, Pubmed ID: 1326551
Skeletal muscle, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-08-22
