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Product Info Summary
| SKU: | A15125 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MRPL49 Antibody
SKU/Catalog Number
A15125
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MRPL49 Antibody (Catalog # A15125). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRPL49 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15125)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Internal of human MRPL49.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A15125 is reactive to MRPL49 in Human
Applications
A15125 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
19.198kDa
Background of MRPL49
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. Pseudogenes corresponding to this gene are found on chromosomes 5q and 8p.
Kas K., Genomics 34:433-436(1996).
Shichijo S., Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HeLa/COLO205 cells, using MRPL49 antibody A15125.
Protein Target Info & Infographic
Gene/Protein Information For MRPL49 (Source: Uniprot.org, NCBI)
Gene Name
MRPL49
Full Name
39S ribosomal protein L49, mitochondrial
Weight
19.198kDa
Superfamily
mitochondrion-specific ribosomal protein mL49 family
Alternative Names
C11orf4; L49mt39S ribosomal protein L49, mitochondrial; mitochondrial ribosomal protein L49; MRP-L49; Neighbor of FAU; next to FAU; NOF1chromosome 11 open reading frame 4; NOFMGC10656; Protein NOF1 MRPL49 C11orf4, L49mt, MRP-L49, NOF, NOF1 mitochondrial ribosomal protein L49 39S ribosomal protein L49, mitochondrial|mitochondrial large ribosomal subunit protein mL49|neighbor of FAU|next to FAU
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRPL49, check out the MRPL49 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRPL49: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRPL49 Antibody (A15125)
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No publications found for A15125
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-MRPL49 Antibody
Question
Can you help my question with product A15125, anti-MRPL49 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A15125 anti-MRPL49 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-09
Question
Does anti-MRPL49 antibody A15125 work for WB with colon adenocarcinoma?
P. Roberts
Verified customer
Asked: 2017-08-24
Answer
According to the expression profile of colon adenocarcinoma, MRPL49 is highly expressed in colon adenocarcinoma. So, it is likely that anti-MRPL49 antibody A15125 will work for WB with colon adenocarcinoma.
Boster Scientific Support
Answered: 2017-08-24
Question
We are currently using anti-MRPL49 antibody A15125 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
The anti-MRPL49 antibody (A15125) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-31
