Mouse CD13/Aminopeptidase N ELISA Kit PicoKine™

Mouse CD13/Aminopeptidase N ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Anpep in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1222
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse CD13/Aminopeptidase N ELISA Kit PicoKine™

See all Aminopeptidase N/CD13 products

SKU/Catalog Number

EK1222

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CD13/Aminopeptidase N ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Anpep in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse CD13/Aminopeptidase N ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1222)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: K69-S966

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1222 is reactive to Anpep in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1222 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1222-CAP Anti-Mouse Anpep Pre-coated 96-well strip microplate 1
EK1222-ST Mouse Anpep Standard 2 vials, 20 ng/tube
EK1222-DA Mouse Anpep Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0200.0550.0660.1200.2310.5020.9181.816

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Aminopeptidase N/CD13, Dilution ratio of 1:50, concentration in serum is around 200ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse CD13/Aminopeptidase N ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6421721936866015509748
Standard deviation50.07134.23543.3457.42127.1633.62
CV (%)7.8%7.8%5.8%8.7%8.2%6.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Aminopeptidase N/CD13 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 164264665268265515.72.3%
Sample 21721152215461514157584.685.3%
Sample 393689521915094459371138.581.4%
*number of samples for each test n=16.

Gene/Protein Information For Anpep (Source: Uniprot.Org, NCBI)

Uniprot ID

P97449

Gene ID

16790

Gene Name

Anpep

Full Name

Aminopeptidase N

Weight

109.651kDa

Superfamily

peptidase M1 family

Alternative Names

alanyl (membrane) aminopeptidase; Alanyl aminopeptidase; Aminopeptidase M; Aminopeptidase N; ANPEP; AP-M; APN; AP-N; CD13 antigen; CD13; CD13APN; EC 3.4.11; EC 3.4.11.2; gp150; LAP1; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; p150; PEPN; PEPNhAPN Anpep|AP-M, AP-N, Apn, Cd13, P150|alanyl (membrane) aminopeptidase|aminopeptidase N|alanyl aminopeptidase|aminopeptidase M|aminopeptidase N/CD13|membrane protein p161|microsomal aminopeptidase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Anpep, check out the Anpep Infographic

Anpep infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Anpep: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1222 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ultrasensitive detection of aminopeptidase N activity in urine and cells with a ratiometric fluorescence probe

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8 Customer Q&As for Mouse CD13/Aminopeptidase N ELISA Kit PicoKine™

Question

Q: Can CD13 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-11-02

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-11-02

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-03-20

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-03-20

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CD13 using the EK1222 Mouse CD13 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-09-17

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CD13 in Mouse cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-02-03

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD13 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-02-03

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2017-10-22

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2017-10-22

Question

Does EK1222 cross-react with rat?

Verified Customer

Verified customer

Asked: 2017-08-16

Answer

EK1222 would not cross react with rat.

Boster Scientific Support

Answered: 2017-08-16

Question

Q: is it okay to use citrate plasma as samples in Mouse CD13 Picokine® ELISA Kit (Catalog # EK1222)?

Verified Customer

Verified customer

Asked: 2017-07-04

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CD13 causing disruption of its protein structure. CD13 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CD13 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2017-07-04

Question

A customer wanted to know if EK1222 cross reacted with rat. Do you have any information?

Verified Customer

Verified customer

Asked: 2015-01-22

Answer

EK1222 would not cross react with rat.

Boster Scientific Support

Answered: 2015-01-22

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