Anti-MRPS22 Antibody

MRPS22 antibody

Boster Bio Anti-MRPS22 Antibody (Catalog # A10300). Tested in WB, IF applications. This antibody reacts with Human.

Product Info Summary

SKU: A10300
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IF, WB

Product Name

Anti-MRPS22 Antibody

View all MRPS22 Antibodies

SKU/Catalog Number

A10300

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MRPS22 Antibody (Catalog # A10300). Tested in WB, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MRPS22 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10300)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MRPS22.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10300 is reactive to MRPS22 in Human

Reconstitution

Calculated molecular weight

41280 MW

Background of MRPS22

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that does not seem to have a counterpart in prokaryotic and fungal-mitochondrial ribosomes. This gene lies telomeric of and is transcribed in the opposite direction from the forkhead box L2 gene. A pseudogene corresponding to this gene is found on chromosome Xq.

Crisponi L., Nat. Genet. 27:159-166(2001).
Li Y., Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10300 is guaranteed for IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For MRPS22 (Source: Uniprot.org, NCBI)

Gene Name

MRPS22

Full Name

28S ribosomal protein S22, mitochondrial

Weight

41280 MW

Superfamily

mitochondrion-specific ribosomal protein mS22 family

Alternative Names

28S ribosomal protein S22, mitochondrial;MRP-S22;S22mt;MRPS22;C3orf5, RPMS22;GK002; MRPS22 C3orf5, COXPD5, GIBT, GK002, MRP-S22, ODG7, RPMS22 mitochondrial ribosomal protein S22 28S ribosomal protein S22, mitochondrial|S22mt|mitochondrial small ribosomal subunit protein mS22

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MRPS22, check out the MRPS22 Infographic

MRPS22 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MRPS22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-MRPS22 Antibody

Question

We are interested in to test anti-MRPS22 antibody A10300 on human brain for research purposes, then I may be interested in using anti-MRPS22 antibody A10300 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-10

Answer

The products we sell, including anti-MRPS22 antibody A10300, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-10

Question

We are currently using anti-MRPS22 antibody A10300 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-18

Answer

The anti-MRPS22 antibody (A10300) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-18

Question

Please see the WB image, lot number and protocol we used for brain using anti-MRPS22 antibody A10300. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-21

Question

Does A10300 anti-MRPS22 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

N. Jackson

Verified customer

Asked: 2018-07-26

Answer

It shows on the product datasheet, A10300 anti-MRPS22 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-07-26

Question

I was wanting to use your anti-MRPS22 antibody for IF for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?

Verified Customer

Verified customer

Asked: 2018-03-22

Answer

You can see on the product datasheet, A10300 anti-MRPS22 antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-03-22

Order DetailsPrice
A10300-100uL

100uL

$360

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A10300
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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