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Product Info Summary
| SKU: | A04922S376 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MSK1 (phospho-S376) RPS6KA5 Antibody
SKU/Catalog Number
A04922S376
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MSK1 (phospho-S376) RPS6KA5 Antibody catalog # A04922S376. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MSK1 (phospho-S376) RPS6KA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04922S376)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around S376 of human RPS6KA5(NP_004746.2).
Reactive Species
A04922S376 is reactive to RPS6KA5 in Human, Mouse, Rat
Observed Molecular Weight
111 kDa
Calculated molecular weight
89.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04922S376 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:50-1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-RPS6KA5-S376 antibody at 1:2000 dilution. NIH/3T3 cells were treated by Anisomycin for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat testis using Phospho-RPS6KA5-S376 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-RPS6KA5-S376 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-RPS6KA5-S376 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Specific Publications For Anti-MSK1 (phospho-S376) RPS6KA5 Antibody (A04922S376)
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