Click image to see more details
Product Info Summary
| SKU: | M01069 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-MST1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01069
BM5671 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MST1 Rabbit Monoclonal Antibody catalog # M01069. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MST1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01069)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFBA-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MST1
Reactive Species
M01069 is reactive to MST1 in Human, Mouse, Rat
Observed Molecular Weight
85 kDa
Calculated molecular weight
80.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01069 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
FC 1:50
Positive Control
WB: HepG2 cell
IHC: human liver cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of MST1 expression in HepG2 cell lysate.
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IHC analysis of MST1 using anti-MST1 antibody (M01069).
MST1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MST1 Antibody (M01069) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-MST1 Rabbit Monoclonal Antibody (M01069)
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Customer Q&As
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9 Customer Q&As for Anti-MST1 Rabbit Monoclonal Antibody
Question
Is this M01069 anti-MST1 Monoclonal antibody reactive to the isotypes of MST1?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
The immunogen of M01069 anti-MST1 Monoclonal antibody is A synthesized peptide derived from human MST1 Probably has no proteolytic activity, since crucial characteristic of serine proteases catalytic sites are not conserved. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-08
Question
I see that the anti-MST1 Monoclonal antibody M01069 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-14
Question
I have attached the WB image, lot number and protocol we used for plasma using anti-MST1 Monoclonal antibody M01069. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-08
Question
What sample was used in your WB analysis of M01069?
Verified customer
Asked: 2019-12-18
Answer
For the Anti-MST1 Rabbit Monoclonal Antibody (M01069), western blot analysis of MST1 expression in HepG2 cell lysate.
Boster Scientific Support
Answered: 2019-12-20
Question
Is the M01069 antibody liquid or lyophilized?
Verified customer
Asked: 2019-12-18
Answer
The Anti-MST1 Rabbit Monoclonal Antibody (M01069) is liquid.
Boster Scientific Support
Answered: 2019-12-18
Question
Would M01069 anti-MST1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Roberts
Verified customer
Asked: 2019-10-02
Answer
It shows on the product datasheet, M01069 anti-MST1 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-02
Question
Will anti-MST1 Monoclonal antibody M01069 work for WB with plasma?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
According to the expression profile of plasma, MST1 is highly expressed in plasma. So, it is likely that anti-MST1 Monoclonal antibody M01069 will work for WB with plasma.
Boster Scientific Support
Answered: 2019-05-14
Question
Do you have a BSA free version of anti-MST1 Monoclonal antibody M01069 available?
Verified Customer
Verified customer
Asked: 2019-02-18
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MST1 Monoclonal antibody M01069 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-18
Question
My lab would like to test anti-MST1 Monoclonal antibody M01069 on human plasma for research purposes, then I may be interested in using anti-MST1 Monoclonal antibody M01069 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-02-14
Answer
The products we sell, including anti-MST1 Monoclonal antibody M01069, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-14
