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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-MST1 Antibody Picoband™
Boster Bio Anti-MST1 Antibody Picoband™ catalog # A01069-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MST1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01069-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence of human MST1 (QRSPLNDFQVLRGTELQHLLHAVVPGPWQEDVADAEE).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A01069-1 is reactive to MST1 in Human, Mouse, Rat
A01069-1 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MST1 using anti-MST1 antibody (A01069-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela cell lysate,
Lane 2: human MCF-7 cell lysate,
Lane 3: human HepG2 cell lysate,
Lane 4: human SK-OV-3 cell lysate,
Lane 5: human A549 cell lysate,
Lane 6: rat kidney tissue lysate,
Lane 7: mouse kidney tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST1 antigen affinity purified polyclonal antibody (Catalog # A01069-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MST1 at approximately 80KD. The expected band size for MST1 is at 80KD.
Gene/Protein Information For MST1 (Source: Uniprot.Org, NCBI)
Hepatocyte growth factor-like protein
peptidase S1 family
D3F15S2; EC 3.4.21; hepatocyte growth factor-like protein homolog; HGFL; HGFLhepatocyte growth factor-like protein; macrophage stimulating 1 (hepatocyte growth factor-like); Macrophage stimulatory protein; Macrophage-stimulating protein; MSP; MSPDNF15S2; MST1; NF15S2; SF2 MST1 D3F15S2, DNF15S2, HGFL, MSP, NF15S2 macrophage stimulating 1 hepatocyte growth factor-like protein|hepatocyte growth factor-like protein homolog|macrophage stimulating 1 (hepatocyte growth factor-like)|macrophage-stimulating protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MST1, check out the MST1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MST1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for A01069-1
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