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- Table of Contents
MST1/STK4 antibody (A00859-2) performs well in ELISA, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of MST1/STK4 using anti-MST1/STK4 antibody (A00859-2).
MST1/STK4 was detected in a paraffin-embedded section of human lymph tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MST1/STK4 Antibody (A00859-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
MST1/STK4 was detected in a paraffin-embedded section of human lymph tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MST1/STK4 Antibody (A00859-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-MST1/STK4 Antibody Picoband®
MST1/STK4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IP, IF, IHC, ICC, WB |
Tested Applications
MST1/STK4 antibody was positively detected by WB in:
human HeLa whole cell, human HepG2 whole cell, human Jurkat whole cell, human Ramos whole cell, rat liver tissue, rat spleen tissue, mouse liver tissue, mouse spleen tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MST1/STK4 antibody was positively detected by IHC in:
human lymph tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
MST1/STK4 antibody was positively detected by ICC/IF in:
Hela cell
MST1/STK4 antibody was positively detected by IP in:
HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Immunoprecipitation
0.5-2 μg/ml
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-MST1/STK4 Antibody Picoband®
View all MST1/STK4 Antibodies
View all MST1/STK4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human MST1/STK4 recombinant protein (Position: E324-D452). Human STK4 shares 93.8% amino acid (aa) sequence identity with mouse STK4.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
56 kDa
Calculated Molecular Weight
55.6 kDa
Background of MST1/STK4
Serine/threonine-protein kinase 4 is an enzyme that in humans is encoded by the STK4 gene. The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.
Cite This Product
Anti-MST1/STK4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00859-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Mouse
94%
High Homology
Cow
94%
High Homology
Sheep
94%
High Homology
Rabbit
93%
High Homology
Rat
92%
High Homology
Dog
92%
High Homology
Zebrafish
50%
Low homology
Drosophila
38%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MST1/STK4 antibody
Immunohistochemistry (IHC) protocol for MST1/STK4 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MST1/STK4 antibody
Immunoprecipitation (IP) protocol for MST1/STK4 antibody
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Product Promise
We promise Anti-MST1/STK4 Antibody Picoband® performs excellently in relevant applications for ELISA, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A00859-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026