Click image to see more details
Product Info Summary
| SKU: | M01119 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-mtTFA Rabbit Monoclonal Antibody
SKU/Catalog Number
M01119
BM5453 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-mtTFA Rabbit Monoclonal Antibody catalog # M01119. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-mtTFA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01119)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADHC-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human mtTFA
Reactive Species
M01119 is reactive to TFAM in Human
Observed Molecular Weight
24 kDa
Calculated molecular weight
29.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01119 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
Positive Control
WB: MCF7 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of mtTFA expression in MCF7 cell lysate.
Specific Publications For Anti-mtTFA Rabbit Monoclonal Antibody (M01119)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-mtTFA Rabbit Monoclonal Antibody
Question
Would anti-mtTFA Monoclonal antibody M01119 work for WB with lymphocyte?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
According to the expression profile of lymphocyte, TFAM is highly expressed in lymphocyte. So, it is likely that anti-mtTFA Monoclonal antibody M01119 will work for WB with lymphocyte.
Boster Scientific Support
Answered: 2020-03-31
Question
Our lab used your anti-mtTFA Monoclonal antibody for IF on hepatoma last year. I am using human, and We intend to use the antibody for IP next. Our lab want to know about examining hepatoma as well as cervix carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
I have checked the website and datasheets of our anti-mtTFA Monoclonal antibody and it appears that M01119 has been tested on human in both IF and IP. Thus M01119 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-27
Question
What sample was used in your WB analysis of M01119?
Verified customer
Asked: 2019-12-18
Answer
For the Anti-MtTFA Rabbit Monoclonal Antibody (M01119), western blot analysis of mtTFA expression in MCF7 cell lysate.
Boster Scientific Support
Answered: 2019-12-20
Question
We are interested in to test anti-mtTFA Monoclonal antibody M01119 on human lymphocyte for research purposes, then I may be interested in using anti-mtTFA Monoclonal antibody M01119 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
The products we sell, including anti-mtTFA Monoclonal antibody M01119, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymphocyte using anti-mtTFA Monoclonal antibody M01119. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-25
Question
I have a question about product M01119, anti-mtTFA Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01119 anti-mtTFA Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-18
Question
We have observed staining in human cervix carcinoma erythroleukemia. Any tips? Is anti-mtTFA Monoclonal antibody supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
According to literature cervix carcinoma erythroleukemia does express TFAM. According to Uniprot.org, TFAM is expressed in female gonad, hepatoma, tongue, lymphocyte, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have TFAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymphocyte, Pubmed ID: 1610904
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-09-12
Question
I see that the anti-mtTFA Monoclonal antibody M01119 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-25
Question
Is a blocking peptide available for product anti-mtTFA Monoclonal antibody (M01119)?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
We do provide the blocking peptide for product anti-mtTFA Monoclonal antibody (M01119). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-11
Question
See below the WB image, lot number and protocol we used for lymphocyte using anti-mtTFA Monoclonal antibody M01119. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-31
Question
Would M01119 anti-mtTFA Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
O. Miller
Verified customer
Asked: 2018-09-05
Answer
You can see on the product datasheet, M01119 anti-mtTFA Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-05
Question
Is this M01119 anti-mtTFA Monoclonal antibody reactive to the isotypes of TFAM?
Verified Customer
Verified customer
Asked: 2018-05-10
Answer
The immunogen of M01119 anti-mtTFA Monoclonal antibody is A synthesized peptide derived from human mtTFA Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-10
Question
We are currently using anti-mtTFA Monoclonal antibody M01119 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
L. Thomas
Verified customer
Asked: 2018-03-06
Answer
The anti-mtTFA Monoclonal antibody (M01119) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-06
Question
My colleagues were happy with the WB result of your anti-mtTFA Monoclonal antibody. However we have observed positive staining in cervix carcinoma erythroleukemia mitochondrion using this antibody. Is that expected? Could you tell me where is TFAM supposed to be expressed?
J. Jones
Verified customer
Asked: 2015-11-04
Answer
From literature, cervix carcinoma erythroleukemia does express TFAM. Generally TFAM expresses in mitochondrion. Regarding which tissues have TFAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymphocyte, Pubmed ID: 1610904
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-11-04
Question
I was wanting to use your anti-mtTFA Monoclonal antibody for WB for human lymphocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymphocyte identification?
P. Taylor
Verified customer
Asked: 2014-03-13
Answer
You can see on the product datasheet, M01119 anti-mtTFA Monoclonal antibody has been validated for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymphocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-03-13
Question
Is there a BSA free version of anti-mtTFA Monoclonal antibody M01119 available?
N. Mangal
Verified customer
Asked: 2013-02-14
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-mtTFA Monoclonal antibody M01119 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-02-14
