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Product Info Summary
| SKU: | M08332 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-MUC3 (Mucin 3) Monoclonal Antibody
SKU/Catalog Number
M08332
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-MUC3 (Mucin 3) Monoclonal Antibody (Catalog # M08332). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-MUC3 (Mucin 3) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08332)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: MUC3/1154
Isotype
IgG2a, kappa
Immunogen
A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M08332 is reactive to MUC3A in Human
Applications
M08332 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
345.127kDa
Background of MUC3
This monoclonal antibody recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). This monoclonal antibody shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with Anti-MUC3 Monoclonal Antibody (MUC3/1154).
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Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with Anti-MUC3 Monoclonal Antibody (MUC3/1154).
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Anti-MUC3 Monoclonal Antibody (MUC3/1154).
Protein Target Info & Infographic
Gene/Protein Information For MUC3A (Source: Uniprot.org, NCBI)
Gene Name
MUC3A
Full Name
Mucin-3A
Weight
345.127kDa
Alternative Names
MUC-3A; mucin 3, intestinal; mucin 3A, cell surface associated; mucin 3A, intestinal; mucin-3A MUC3A MUC-3A, MUC3 mucin 3A, cell surface associated mucin-3A|intestinal mucin|mucin 3, intestinal
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MUC3A, check out the MUC3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MUC3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MUC3 (Mucin 3) Monoclonal Antibody (M08332)
Hello CJ!
No publications found for M08332
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-MUC3 (Mucin 3) Monoclonal Antibody
Question
We are currently using anti-MUC3 (Mucin 3) Monoclonal antibody M08332 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
The anti-MUC3 (Mucin 3) Monoclonal antibody (M08332) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-01
